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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 255 - 262 (8page)
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초록· 키워드
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Plasmid pCAMBIA2201-pta, containing the selectable marker neomycin phosphotransferase gene (npt II), Pinellia ternata agglutinin (PTA) gene and β-glucuronidase gene, were transformed into leaf discs of Chrysanthemum (Chrysanthemum morifolium Ramat.) via Agrobacterium tumefaciens-mediated transformation. It was confrmed by GUS (β-glucuronidase) and PCR analysis that the PTA gene had been successfully inserted into the plant genome. Hemagglutination assay was carried out and the expression of PTA gene in transgenic plants in vitro and the aphid-resistance assay result showed that the inhibition rates of the transformed plants were improved than the control. T1 progenies generated through the cuttage breeding from T0 generation had preserved the aphid-resistance and the inhibition rates of T1 generation had no signifcant diference with T0 generation (P>0.05). The results suggest that PTA gene can improve aphid resistance of chrysanthemum and its aphid resistance can be generated to T1 generation stably.
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참고문헌 (33)
참고문헌 신청
Davies FT / 2004 / Fertility affects susceptibility of chrysanthemum to cotton aphids: influence on plant growth, photosynthesis, ethylene evolution, and herbivore abundance / J Am Soc Hortic Sci 129:344~353
Stephen L. Dellaporta / 1983 / A plant DNA minipreparation: Version II / Plant Molecular Biology Reporter 1(4):19~21
Duan X / 2018 / Expression of Pinellia pedatisecta lectin gene in transgenic wheat enhances resistance to wheat aphids / Molecules 23:748
Xiao Fu / 2018 / Genetic variation and association mapping of aphid (Macrosiphoniella sanbourni) resistance in chrysanthemum (Chrysanthemum morifolium Ramat.) / Euphytica 214(2):21
S. Fukai / 1995 / AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION OF CHRYSANTHEMUM / Acta Horticulturae (392):147~152
Stephen L. Dellaporta / 1983 / A plant DNA minipreparation: Version II / Plant Molecular Biology Reporter 1(4):19~21
Duan X / 2018 / Expression of Pinellia pedatisecta lectin gene in transgenic wheat enhances resistance to wheat aphids / Molecules 23:748
Xiao Fu / 2018 / Genetic variation and association mapping of aphid (Macrosiphoniella sanbourni) resistance in chrysanthemum (Chrysanthemum morifolium Ramat.) / Euphytica 214(2):21
S. Fukai / 1995 / AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION OF CHRYSANTHEMUM / Acta Horticulturae (392):147~152
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