지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
To evaluate the toxicity of ophthalmic drug, the Draize test and Bovine Corneal Opacity and Permeability (BCOP) test commonly used. In Draize test, experimental animals were under stress and pain due to long-term exposure of drug. In addition, regarding physiological functions, animal model is not perfectly reflected a human eye condition. Although some models such as EpiOcularTM, HCE model, LabCyte Cornea-Model, and MCTT HCETM were already presented advanced cornea ex-vivo model to replace animal test. In this sense, cornea tissue structure mimicked ex-vivo toxicity model was fabricated in this study. The corneal epithelial cells (CECs) and keratocytes (CKs) isolated from rabbit eyeball were seeded on non-patterned silk film (n-pSF) and patterned silk film (pSF) at 32,500 cells/cm2 and 6,500 cells/cm2. Sequentially, n-pSF and pSF were stacked to mimic a multi-layered stroma structure. The thickness of films was about 15.63 μm and the distance of patterns was about 3 μm. H&E stain was performed to confirm the cell proliferation on silk film. F-actin of CKs was also stained with Phalloidin to observe the cytoskeletal alignment along with patterns of the pSF. In the results, CECs and CKs were shown the good cell attachment on the n-pSF and pSFs. Proliferated cells expressed the specific phenotype of cornea epithelium and stroma. In conclusion, we successfully established the ex-vivo cornea toxicity model to replace the eye irritation tests. In further study, we will set up the human ex-vivo cornea toxicity model and then will evaluate the drug screening efficacy.
목차
등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
DelMonte DW / 2011 / Anatomy and physiology of the cornea / J Cataract Refract Surg 37(3):588~598
Dohlman CH / 1971 / The function of the corneal epithelium in health and disease / Invest Ophthalmol 10(6):381~407
Draize JH / 1944 / Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes / J Pharmacol Exp Ther 82(3):377~390
Wilhelmus KR / 2001 / The Draize eye test / Surv Ophthalmol 45(6):493~515
Vinardell M / 2008 / Alternative methods for eye and skin irritation tests : an overview / J Pharm Sci 97(1):46~59
Dohlman CH / 1971 / The function of the corneal epithelium in health and disease / Invest Ophthalmol 10(6):381~407
Draize JH / 1944 / Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes / J Pharmacol Exp Ther 82(3):377~390
Wilhelmus KR / 2001 / The Draize eye test / Surv Ophthalmol 45(6):493~515
Vinardell M / 2008 / Alternative methods for eye and skin irritation tests : an overview / J Pharm Sci 97(1):46~59
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