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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
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Background: In the present study, we evaluated four commonly used housekeeping genes, viz., actin-β, elongation factor-1α (EF1α), acidic ribosomal protein (ARP), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as internal references for quantitative analysis of immune genes in nervous necrosis virus (NNV)-infected seven-band grouper, Hyporthodus septemfasciatus. Methods: Expression profiles of the four genes were estimated in 12 tissues of healthy and infected seven-band grouper. Expression stability of the genes was calculated using the delta Ct method, BestKeeper, NormFinder, and geNorm algorithms. Consensus ranking was performed using RefFinder, and statistical analysis was done using GraphpadPrism 5.0. Results: Tissue-specific variations were observed in the four tested housekeeping genes of healthy and NNVinfected seven-band grouper. Fold change calculation for interferon-1 and Mx expression using the four housekeeping genes as internal references presented varied profiles for each tissue. EF1α and actin-β was the most stable expressed gene in tissues of healthy and NNV-infected seven-band grouper, respectively. Consensus ranking using RefFinder suggested EF1α as the least variable and highly stable gene in the healthy and infected animals. Conclusions: These results suggest that EF1α can be a fairly better internal reference in comparison to other tested genes in this study during the NNV infection process. This forms the pilot study on the validation of reference genes in Hyporthodus septemfasciatus, in the context of NNV infection.
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참고문헌 (34)
참고문헌 신청
Andersen CL / 2004 / Normalization of real-time quantitative reverse transcription-PCR data : a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets / Cancer Res 64(15):5245~5250
Bustin SA / 2000 / Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays / J Mol Endocrinol 25(2):169~193
Bustin SA / 2005 / Quantitative real-time RT-PCR-a perspective / J Mol Endocrinol 34(3):597~601
Casadei R / 2011 / Identification of housekeeping genes suitable for gene expression analysis in the zebrafish / Gene Expression Patterns 11(3-4):271~276
Dheda K / 2004 / Validation of housekeeping genes for normalizing RNA expression in real-time PCR / Biotechniques 37(1):112~119
Bustin SA / 2000 / Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays / J Mol Endocrinol 25(2):169~193
Bustin SA / 2005 / Quantitative real-time RT-PCR-a perspective / J Mol Endocrinol 34(3):597~601
Casadei R / 2011 / Identification of housekeeping genes suitable for gene expression analysis in the zebrafish / Gene Expression Patterns 11(3-4):271~276
Dheda K / 2004 / Validation of housekeeping genes for normalizing RNA expression in real-time PCR / Biotechniques 37(1):112~119
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Prevention strategy for viral nervous necrosis (VNN) in the aquaculture farm of sevenband grouper (Epinephelus septemfasciatus)
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Structural organization of genes encoding prolactin in hybrid Red-spotted grouper (Epinephelus akaara) and Giant grouper (E. lanceolatus) and their expression
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Effect of thermal optima on cell mediated immune responses and virus induced cellular senescence following nervous necrosis virus infection in sevenband grouper
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EARLY OSTEOLOGICAL DEVELOPMENT OF THE LARVAE AND JUVENILE IN SEVENBAND GROUPER Epinephelus septemfasciatus
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