지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The culture of the intestinal epithelium into three dimensional (3D) structures typically termed organoid culture. Organoid culture is based on the ability of intestinal stem cells (ISCs), at the base of the crypt, perpetually to divide and produce a fully differentiated, polarized epithelium. Leucine-rich-repeat-containing G-protein-coupled receptor 5 (Lgr5) positive ISCs isolated from the intestine can form organoids in long-term culture. Thus, when cultured under the appropriate 3D conditions, single Lgr5+ ISCs undergo cycles of self-renewal, differentiation and morphogenesis, and self-organize into crypt-villus domains that house cycling ISCs and differentiated intestinal epithelial cells (IECs). In this study, we performed isolation, characterization and consecutive subculture of small intestinal crypts from BALB/c-nude mouse. Briefly, isolated mouse crypts were embedded in matrigel, cast into 40 μL droplets at the bottom of well in a 48-well plate. Following polymerization, the gels were overlaid with ISCs expansion medium containing B27, N2, N-acetylcysteine, epidermal growth factor, noggin, and R-spondin 1. As a result, mouse crypt-derived ISCs had enteroids and spheroid morphologies. We also confirmed by quantitative real-time RT-PCR that expression of ISCs-related specific genes (Lgr5, sox9) and IECs-related specific genes (chromogranin A, defensin-5, mucin-1, mucin-2, and villin) was maintained at eight passages or more. Thus, we observed that expression of specific markers and consecutive self-renewing in the mouse small intestinal crypt-derived organoids.
목차
등록된 정보가 없습니다.
참고문헌 (37)
참고문헌 신청
Barkauskas CE / 2017 / Lung organoids : current uses and future promise / Development 144:986~997
Barker N / 2007 / Identification of stem cells in small intestine and colon by marker gene Lgr5 / Nature 449:1003~1007
Broutier L / 2017 / Human primary liver cancer-derived organoid cultures for disease modeling and drug screening / Nat Med 23:1424~1435
Bry L / 1994 / Paneth cell differentiation in the developing intestine of normal and transgenic mice / Proc Natl Acad Sci USA 91:10335~10339
Chen S / 2018 / Glutamine supplementation improves intestinal cell proliferation and stem cell differentiation in weanling mice / Food Nutr Res 62:1439
Barker N / 2007 / Identification of stem cells in small intestine and colon by marker gene Lgr5 / Nature 449:1003~1007
Broutier L / 2017 / Human primary liver cancer-derived organoid cultures for disease modeling and drug screening / Nat Med 23:1424~1435
Bry L / 1994 / Paneth cell differentiation in the developing intestine of normal and transgenic mice / Proc Natl Acad Sci USA 91:10335~10339
Chen S / 2018 / Glutamine supplementation improves intestinal cell proliferation and stem cell differentiation in weanling mice / Food Nutr Res 62:1439
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
[S16-4] Use of human/animal-derived intestinal stem cells and their differentiated cells to mimic intestinal metabolism and physiology
한국실험동물학회 학술발표대회 논문집
2024 .07
-
Histological Architecture of Gastric Epithelial Neoplasias That Showed Absent Microsurface Patterns, Visualized by Magnifying Endoscopy with Narrow-Band Imaging
Clinical Endoscopy
2021 .01
-
Expression of Leucine-rich Repeat-containing G-protein Coupled Receptor 5 and CD44: Potential Implications for Gastric Cancer Stem Cell Marker
대한암예방학회지
2016 .01
-
Prevalence of Abnormal Urodynamic Study Results in Patients with Congenital and Idiopathic Scoliosis and Its Predictive Value for the Diagnosis of Tethered Cord Syndrome: A Single Institution Clinical Study
Asian Spine Journal
2021 .01
-
Human pluripotent stem cell-derived 3D intestinal organoids and their applications
한국실험동물학회 학술발표대회 논문집
2017 .08
-
Evaluation of the regenerative potential of cell therapeutics using repair-associated cell-containing intestinal organoid
한국실험동물학회 학술발표대회 논문집
2022 .07
-
Past, present, and future of Intestinal Research
Intestinal research
2023 .01
-
Clinical relevance of Lgr5 expression in colorectal cancer patients
KOREAN JOURNAL OF CLINICAL ONCOLOGY
2018 .12
-
Stem cell spheroids loaded with quercetin microparticles exert potent protective effects against murine model and induce the generation of wound-healing epithelial cells in intestinal organoids
한국실험동물학회 학술발표대회 논문집
2020 .07
-
Comparative analysis of bisphenol A effects on HT-29 cell line and mouse intestinal organoids
Journal of Biomedical and Translational Research
2024 .09
-
Enteroendocrine cell-derived hormone A is involved in the intestinal homeostasis by directing the differentiation of the mouse intestinal organoid
한국실험동물학회 학술발표대회 논문집
2019 .07
-
Development of mouse organoid-based gastric carcinogenesis models
한국실험동물학회 학술발표대회 논문집
2019 .01
-
Organoids as a New In Vitro Model of Human Norovirus Infection
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2020 .09
-
The establishment of gastric organoids derived from functionally heterogenous epithelium of the mouse stomach
한국실험동물학회 학술발표대회 논문집
2017 .02
-
[Nutrition & Metabolism] Outcome of Intestinal Rehabilitation for Adult Patients with Intestinal Failure : A 20-Year Single Center Experience
대한외과학회 학술대회 초록집
2022 .11
-
Organoid-based study of epithelial homeostasis and regeneration
한국실험동물학회 학술발표대회 논문집
2020 .07
-
Vitamin D Improves Intestinal Barrier Function in Cirrhosis Rats by Upregulating Heme Oxygenase-1 Expression
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Gastric organoid can mimic function in stomach of mouse
한국실험동물학회 학술발표대회 논문집
2016 .08
-
Celecoxib, a selective cyclooxygenase-2 inhibitor, aggravates radiation-induced intestinal damage in mice
Journal of Biomedical and Translational Research
2021 .01
-
Organoids: A new Platform in basic and translational Reserach
한국실험동물학회 학술발표대회 논문집
2021 .07
이 논문의 저자 정보
최근 본 자료
댓글(0)
0