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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국동물위생학회(구 한국가축위생학회) 한국가축위생학회지 한국가축위생학회지 제43권 제2호
발행연도
2020.1
수록면
107 - 112 (6page)

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초록· 키워드

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Foot-and-mouth disease (FMD) and avian influenza (AI) are highly pathogenic viral disease which af-fects the livestock industry worldwide. Outbreak of these viruses causes great impact in the livestock industry; thus, disease infected animals were immediately disposed. Burial is the commonly used dis-posal method for deceased animals. However, there is potential for secondary environmental con-tamination, as well as the risk that infectious agents persisting in the environment due to the limited environmental controls in livestock burial sites during the decomposition of the carcasses. Therefore, this study aimed to investigate the detection of FMD and AI viruses from animal carcass disposal sites using real-time reverse transcription PCR. Soil samples of more than three years post-burial from livestock carcass disposal sites were collected and processed RNA isolation using a commercial extraction kit. The isolated RNA of the samples was used for the detection of FMDV and AIV using qRT-PCR. Based on the qPCR assay result, no viral particle was detected in the soil samples collected from the animal disposal sites. This indicates that 3 years of burial and their carcass disposal method is efficient for the control or at least reduction of spread infections in the surrounding environment.

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