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논문 기본 정보

자료유형
학술대회자료
저자정보
Kim, Kwang-Sig (National Livestock Research Institute) Kim, Chang-Keun (Department of Animal Sci & Technology, Chung Ang University) Chung, Yung-Chai (Department of Animal Sci & Technology, Chung Ang University) Hwang, Seong-Soo (Department of Animal Sci & Technology, Chung Ang University) Park, Jin-Ki (National Livestock Research Institute) Chang, Won-Kyong (National Livestock Research Institute)
저널정보
한국동물번식학회 한국동물번식학회 학술대회 한국동물번식학회 2001년도 춘계학술발표대회
발행연도
2001.1
수록면
28 - 28 (1page)

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이 논문의 연구 히스토리 (2)

2001

Effect of EGF on In Vitro Oocyte Maturation and Embryo Development and Expression of EGF mRNA in Bovine Oocytes and Embryo I. Influence of Cumulus Expression and Maturation and Embryo Development during Bovine Oocyte Maturation In vitro by Addition EGF
Kim ,  Kwang-Sig ,  Kim 외 10명 한국동물번식학회 학술대회 2001.01 학술대회자료
Effect of EGF on In Vitro Oocyte Maturation and Embryo Development and Expression of EGF mRNA in Bovine Oocytes and Embryo II. Detection of Epidermal Growth Factor mRNA in bovine Ova during In Vitro Maturation and after Fertilization In Vitro
Kim ,  Kwang-Sig ,  Kim 외 16명 한국동물번식학회 학술대회 2001.01 학술대회자료

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The objective of this study was to the effect on subsequent development of EGF present in defined medium during bovine 1)oocyte maturation or 2)embryo culture. The presence of EGF during IVM, irrespective of concentration(1, 10, 100ng/$m\ell$), stimulated cumulus expansion and significantly increased the proportion of oocytes attaining metaphaseII, the rate of cleavage, and develop to blastocyst. 1. In the group of EGF-added medium(1, 10, 100ng/$m\ell$), nuclear maturation rate for in vitro maturation was 91% to 92% but was not significantly higher than control group(87%). 2. For in vitro maturation, in the group of EGF-added medium(1, 10, 100ng/$m\ell$)the rate of cumulus cell expansion degree 2 ranged from 81% to 87%, which was significantly higher than the control group(medium with EGF not added). The rate of in vitro fertilization, developing to 2-to 4- cell stage, was 76% to 80%, which was also significantly higher(p<0.05)than control group(62%). 3. For in vitro maturation, in the group of EGF added in medium(1, 10, 100ng/$m\ell$)the development rate to blastocyst was 24.3% to 27%, which was significantly higher than control group(13.7%). The total cleavage rate in the group of EGF-added medium was 77% to 82%, which was higher than control group. 4. The development rate to blastocyst for 6 days of cultivation and the hatching blastocyst were 30.6% and 59.1%, respectively, in the group of 100ng/$m\ell$ of EGF, which were significantly higher(p<0.05)than control group(14.0% and 24%, respectively), The numbers of cells in blastocyst were 140.2 and 148, respectively, in 10ng/$m\ell$ and 100ng/$m\ell$ of EGF-added medium, which were higher than 108.5 in control group. 5. The development rate of in vitro fertilized embryos to blastocyst in 10ng/$m\ell$ of EGF-added medium co-cultured with somatic cell was 28%, which was significantly higher(p<0.05)than control group(11.8%). The numbers of cells in blastocyst were 141.6 for EGF-added medium and 145 for EGF+co-culture group, which were higher than control(101.6)and medium co-cultured with somatic cells(110.6). These results showed that in vitro maturation and fertilization, EGF was found a significant effect of increase of development rate to blastocyst and cell number.
#EGF #cumulus cell expansion #metaphase II #cell number

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