Systems for Production of Calves from Hanwoo IVM/IVF/IVC Blastocyst. IV. Direct Transfer of Vitrified and One-Step Diluted Hanwoo Blastocysts

김은영; 박세필; 김덕임; 이문걸; 이종우; 이금실; 박세영; 박은미; 윤지연

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자료유형: 학술대회자료

저자정보: 김은영 (마리아 기초의학연구소/생명공학연구소) 박세필 (마리아 기초의학연구소/생명공학연구소) 김덕임 (농협중앙회 가축개량사업소) 이문걸 (농협중앙회 가축개량사업소) 이종우 (농협중앙회 가축개량사업소) 이금실 (마리아 기초의학연구소/생명공학연구소) 박세영 (마리아 기초의학연구소/생명공학연구소) 박은미 (마리아 기초의학연구소/생명공학연구소) 윤지연 (마리아 기초의학연구소/생명공학연구소)

저널정보: 한국동물번식학회 한국동물번식학회 학술대회 한국동물번식학회 2001년도 춘계학술발표대회

발행연도: 2001.1

수록면: 73 - 73 (1page)

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2001

Systems for Production of Calves from Hanwoo IVM/IVF/IVC Blastocyst. IV. Direct Transfer of Vitrified and One-Step Diluted Hanwoo Blastocysts

김은영 , 박세필 , 김덕임 외 6명 한국동물번식학회 학술대회 2001.01 학술대회자료

Systems for Production of Calves from Hanwoo IVM/IVF/IVC Blastocyst;IV. Direct Transfer of Vitrified and One-Step Diluted Hanwoo Blastocysts

김은영 , 박세필 , 김덕임 외 12명 대한생식의학회 학술대회 2001.01 학술대회자료

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This study was to examine whether the vitrified, one-step diluted and direct transferred Hanwoo IVM/IVF/IVC blastocysts can be successfully survived in vivo and they were succeeded into the live birth. For vitrification, blastocysts were serially exposed in glycerol (G) or/and ethylene glycol (EG) mixtures [10% (v/v) G for 5 min, 10% G plus 20% EG (v/v) for 5 min, and 25% G plus 25% EG (v/v) for 30 sect] which is diluted in 10% FBS added D-PBS. Thawing of straw was carried out in air for 10 sec and then in water bath of $25^{\circ}C$ for 20 sec. One-step dilution within the straw was done in water bath of $25^{\circ}C$ for 1 min. Vitrified and one-step diluted embryos were directly transferred into 36 (natural or hormone induced synchronized) recipient cows in 6 areas of Kyungsang Buk-Do. Pregnancies were confirmed at first when recipient cows did not return to the subsequent estrus cycle, and later by manual palpation per rectum on day 45, 90 and then living calves were derived into parturition. Overall pregnancy was 33.3%(12/36), However, higher pregnancy was obtained when the recipients exhibited estrus one day earlier than the age of transferred embryos (53.3 vs 25.0-27.3%), irrespective of synchronization methods. Also, parous recipients became pregnant higher than nulliparous heifers, And, there were not different in pregnancy rates by the aspect of corpus luteum (CL) quality of recipients (good, 29.4; fair, 37.5; poor, 33.3%). One hundred eight of frozen-thawed Hanwoo blastocysts were directly transferred into 36 recipient cows. In 12 of pregnant cows, 3 cows were aborted and 9 cows were calved [single, 66.7% (6/9): twin, 33.3% (3/9)]. Total embryo implantation rate was 11.1% (12/108). However, 9 Hanwoo calves were lived. Therefore, these results demonstrate that direct transfer technique of vitrified and one-step diluted bovine blastocysts can be applied easily and effectively with the higher pregnancy rate on field trial without the equipment and embryological skills.

#Hanwoo IVM/IVF/IVC blastocyst #Vitrification #One-step dilution #In vitro/In vivo survival #Pregnancy

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Kim Eun-Young

소속기관 Maria Infertility Hospital Medical Institute/Maria Biotech

주요연구분야 농수해양학 > 축산학

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소속기관 마리아기초의학연구소

주요연구분야 의약학 > 임상의학 > 산부인과학 복합학 > 과학기술학

논문수 45 이용수 449

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소속기관 농협중앙회 한우개량사업소