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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2002.1
- 수록면
- 91 - 91 (1page)
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초록· 키워드
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There are many methods to introduce exogenous DNA into embryo for the purpose of producing transgenic animals. Exogenous gene can be integrated into oocyte as a form of sperm vector. In this study, sperm was used as a vector for transgene that is enhanced green fluorescent protein (EGFP). The objective of this study was to investigate the expression of exogenous gene in bovine embryos after injection of spermatozoa cocultured with EGFP fragment. Spermatozoa were plunged into liquid nitrogen and thawed several times or shaked in 0.2% Triton X-100 to remove sperm membrane which followed by DTT treatment. (omitted)
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Transfer of Porcine Embryos Injected with Sperm Carrying with Exogenous DNA
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Uptake of exogenous DNA by using liposome in boar spermatozoa
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Expression of GFP Gene in the Porcine Preimplantation Embryos after ICSI with DNA/Sperm Complex
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Tetracycline-mediated control of EGFP transgene expression in transgenic chicken
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정자미세주입술에 의하여 동결 융해 부고환 정자와 수정시킨 활성화처리 난자의 체외발생율에 관한 연구
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Effect of Sperm Heat Stress on Embryo Development in Cattle
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Development of Bovine Embryos Produced by Intracytoplasmic Sperm Injection (ICSI)
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