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논문 기본 정보

자료유형
학술저널
저자정보
윤우식 (한국과학기술연구원 부설 생명공학연구소) 이철상 (한국과학기술연구원 부설 생명공학연구소) 방남수 (연변대학농학원 축산학부) 구덕본 (한국과학기술연구원 부설 생명공학연구소) 한용만 (한국과학기술연구원 부설 생명공학연구소) 신상태 (충남대학교 수의과대학) 유욱준 (한국과학기술원 생물과학과 생의학연구소) 박창식 (충남대학교 농과대학)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제21권 제4호
발행연도
1997.1
수록면
373 - 379 (7page)

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초록· 키워드

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This study was carried out to determine the hormone treatment scheme for an efficient superovulation and optimal recovery time for obtaining pronuclear embryos suitable for DNA injection in Korean native goats. For a superovulation, FSH(5.6mg) was given over four days in twice daily injections with (FSH/hCG group) or without(FSH group) hCG(100 IU) co-injection at the time of 7th FSH injection. Estrus cycle was synchronized by norgestomet ear-implantation for 11 days and its removal at the time of 6th FSH injection. Among the treated goats, the percentage of ovulated goats, which were examined at 70 to 76 h following implant removal, was greater in FSH/hCG group than in FSH group (100% vs 36.4%) but there was no significant difference in the mean numbers of ovulation points and fertilization rates between the two groups. To optimize hCG treatment scheme and recovery time, we injected hCG at the time of 7th (FSH/hCGa) or 8th(FSH/hCGb) FSH injection and then examined the developmental stage of the embryos recovered at different times after implant removal. In FSH/hCGa group, significant portions(31 to 44%) were beyond 1-cell stage, which was non-injectable, irrespective of their recovery time. However, in FSH/hCGb group recovered at 70 to 76 h after implant removal, great portions(69%) were fertilized and most of them(96.6%) were injectable 1-cell stage. Considering together the fertilization rate and developmental stage of recovered embryos, it is recommendable to administrate hCG at the time of final 8th FSH injection and collect the embryos at 70 to 76 h after implant removal to obtain injectable embryos as many as possible in Korean native goats.

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