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자료유형
학술저널
저자정보
Lee, Kyung-Ok (Department of Moecular Genetics, Seoul Medical Science institute) Heo, Jeong-Ho-Ho (Department of Moecular Genetics, Seoul Medical Science institute) Kim, Hye-Jin (Immunology Laboratory, Severance Hospital, Yonsei University) Lee, Eun-Mi (Immunology Laboratory, Severance Hospital, Yonsei University) Hong, Sung-Hoi (Department of Moecular Genetics, Seoul Medical Science institute) Kim, Yoon-Jung (Department of Moecular Genetics, Seoul Medical Science institute)
저널정보
한국통합생물학회 Korean journal of biological sciences Korean journal of biological sciences 제1권 제1호
발행연도
1997.1
수록면
129 - 134 (6page)

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HLA-A2 is one of the most diversified HLA-class I antigen with 17 subtypes so far identified at the molecular level. HLA-A*02 subtyping has significant implications on the tissue typing for organ and bone marrow transplantations. Recently, DNA-based typing methods have been successfully applied to the elucidation of HLA gene polymorphisms. In the present study, HLA-A*O2 genotyping was established by using nested polymerase chain reaction-sequence specific primers (PCR-SSP) and distribution of A*O2 alleles were determined in Korean individuals. Genomic DNA prepared from four B-lymphoblastoid cell lines and lymphocytes from serologically defined 48 HLA-A2 Korean individuals by phenol/chloroform extractions was typed. The results of the four B-lymphoblastoid cells were consistent with the previous data typed by PCR analysis. Five A*O2 alleles-A*0201, A*0203, A*0206, A*0207 and A*0210-were commonly observed in a total of 17 A*02 alleles. Of these, A*0207 (f=49.0%) was the most frequent allele in Korean population. A*0206 (f=28.3%) and A*0201 (f=17.0%) were also found frequently while A*0203 and A*0210 types were observed in less than 5%. In conclusion, the high level of discrimination for HLA-A*O2 alleles will prove useful and informative in the study of transplant survival, and may identify the importance of allelic differences not readily detectable by serology on host and donor compatibility.

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