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논문 기본 정보

자료유형
학술저널
저자정보
정장용 (진주산업대학교)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제12권 제2호
발행연도
1997.1
수록면
189 - 194 (6page)

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The ovaries of Korean native cows or heifers were obtained from a slaughter house and kept on 28~3O˚C and transported to laboratory within 2 hrs. The follicular oocytes were collected follicles. The oocytes were matured in vitro for 24 hrs. In TCM-199 supplemented with 35 $\pi$g /ml FSH, 10 $\pi$g /ml LH, 1 $\pi$g /ml estradiol-17 and granulosa cells at 39˚C under 5% $CO_2$ in air. The caudal epididymis of Korean native bulls were obtained from a slaughter house and transported to laboratory within 30 minutes. Swim-up of collected spermatozoa and freezing sperm was layered under 2ml fertilization B. 0. medium in two tissue culture tubes and held at a 45˚C angle for 0~2 hrs. They wrer fertilized in vitro by freezing sperm treated with heparin for 24 hrs, and then the zygotes were co-cultured in vitro with bovine oviductal epithelial cells for 7 to 9 days. The follicular oocytes recovered were classified into 41.7% as grade I, 51.5% as grade II and 6.8% as graed III. The number of oocytes recovered per ovary was averaged 8.3 and they were classifed into 2.3 as grade I, 2.5 as grade II and 2.3 as grade III. The cleavage rate of matured oocytes was significantly(P<O.O5) higher after in vitro fertilization in caudal epididymis(66.1%) than freezing sperm(51.3%). Similarly, the proportion of cleaved zygotes that developed to blastocysts was 19.6 and 14.3% respectively. The cleavage rate and in vitro developmental rate in swin-up period of freezing sperm was similar(59.3~ 63.4 and 13.6~ 19.3%).

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