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논문 기본 정보

자료유형
학술저널
저자정보
박병권 (충남대학교 농과대학)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제22권 제2호
발행연도
1998.1
수록면
119 - 126 (8page)

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초록· 키워드

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This study was conducted to find out the recovery rate of oocyte according to the different size of follicles from porcine ovaries, and the effect of in vitro maturation of porcine immature oocyte at the different transportation temperature of ovaries from slaughter house. The results obtained were summarized as follows : 1. The number of follicles per ovary was 22.5. The number of A-and B-typed oocytes(type A: cumulus-enclosed oocyte, type-B : corona-enclosed oocyte) per ovary was 2.4. The proportion of A-and B-typed oocytes was 29.6% of the total recovery oocytes. 2. When the immature oocytes were cultured for 36, 40, 44 and 48 h at 5$^{\circ}C$ transportation temperature of ovary, the germinal vesicle breakdown(GVBD) rates of porcine oocytes were 32.5, 28.2, 22.6 and 25.9% respectively. There were no significant differences between all the culture time for GVBD. Especially, most of oocytes were observed to arrest the development beyond germinal vesicle(GV) stage. 3. When the immature oocytes were cultured for 36, 40, 44 and 48 h at $25^{\circ}C$ transportation temperature of ovary, the GVBD rates were 81.0, 90.0, 91.7 and 92.9%, and the maturation (Met-II) rates were 51.2, 78.8, 76.2 and 78.6%, respectively. 4. When the immature oocytes were cultured for 36, 40, 44 and 48 h at 38$^{\circ}C$ transportation temperature of ovary, the GVBD rates were 93.9, 96.5, 96.5 and 95.3%, and the maturation rates were 62.2, 88.4, 84.7 and 86.0%, respectively. 5. The above results showed that the maturation rates of immature oocytes between $25^{\circ}C$ and 38$^{\circ}C$ transportation temperature of ovary did not differ significantly.

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