Immunogenicity Study of Recombinant Human Basic Fibroblast Growth Factor

Kim, Dong-Hwan; Cho, Hyeon; Kang, Kyung-Koo; Ahn, Byoung-Ok; Kang, Soo-Hyung; Kim, Won-Bae

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자료유형: 학술저널

저자정보: Kim, Dong-Hwan (Research Laboratories, Dong-A Pharm. Co. Ltd.) Cho, Hyeon (Research Laboratories, Dong-A Pharm. Co. Ltd.) Kang, Kyung-Koo (Research Laboratories, Dong-A Pharm. Co. Ltd.) Ahn, Byoung-Ok (Research Laboratories, Dong-A Pharm. Co. Ltd.) Kang, Soo-Hyung (Research Laboratories, Dong-A Pharm. Co. Ltd.) Kim, Won-Bae (Research Laboratories, Dong-A Pharm. Co. Ltd.)

저널정보: 한국응용약물학회 The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 제7권 제1호

발행연도: 1999.1

수록면: 14 - 21 (8page)

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The immunogenicity of the recombinant human basic fibroblast growth factor (rh-bFGF) was investigated by tests for active systemic anaphylaxis (ASA), passive cutaneous anaphylaxis (PCA), passive hemagglutination (PHA) and guinea pig maximization test (GPMT) in mice or guinea pigs. Guinea pigs were sensitized with rh-bFGF ($100-1000\;\mu\textrm{g}/kg$) or rh-bFGF-CFA mixture ($1000\;\mu\textrm{g}/kg$). All animals sensitized with rh-bFGF alone or mixture with CFA showed symptoms of anaphylactic shock. IgE antibodies to rh-bFGF were detected in sera obtained from rh-bFGF and rh-bFGF-Alum ($1000\;\mu\textrm{g}/kg$) sensitized mice, indicating that rh-bFGF has immunogenicity eliciting potential. IgG and/or IgM antibodies to rh-bFGF were also detected in all the sera obtained from sensitized mice by PHA. In the GPMT for delayed type skin reaction, no skin reaction was observed in sensitized guinea pigs after intradermal injection and dermal application of 0.01% rh- bFGF. However, these positive reactions were consistent with the results of another rh-bFGF, showing that rh- bFGF is a heterogenous protein to rodents. Considering the fact that rh-bFGF is a genuine human protein of which structure is identical to the endogenous human bFGF, it is thought that rh-bFGF is rarely associated with immunological problems in clinical use.

#Recombinant Human basic Fibroblast Growth Factor #Immunogenicity #Guinea Pig Maximization Test

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소속기관 서울대학교 약학대학 약제학과

주요연구분야 의약학 > 약학

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Cho, Hyeon