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Subject

Molecular Identification of Wolbachia Naturally Infected in Thecodiplosis japonensis (Diptera: Cecidominideii)
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논문 기본 정보

Type
Academic journal
Author
Kim, Mi-Gwang (Insect Resources Laboratory, Korea Research Institute of Bioscience and Biotechnology) Oh, Hyun-Woo (Insect Resources Laboratory, Korea Research Institute of Bioscience and Biotechnology) Park, Hee-Moon (Department of Microbiology, Chungnam National Universiy) Park, Ho-Yong (Insect Resources Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Journal
한국곤충학회 한국곤충학회지 한국곤충학회지 제30권 제3호 KCI Accredited Journals
Published
2000.1
Pages
139 - 146 (8page)

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Molecular Identification of Wolbachia Naturally Infected in Thecodiplosis japonensis (Diptera: Cecidominideii)
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Abstract· Keywords

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The presence and phylogenetic position of endosymbiotic bacteria were studied from the pine needle gall midge, Thecodiplosis japonensis that is the most serious insect pest of the red pine, Pinus densif lora. PCR amplification using the general primers for eubacterial16S ribosomal DNA (rDNA) and the sequence of the amplified product showed that T. japonensis harbours the obligate intracellular bacteria Wolbachia, which has been known to infect an extremely wide range of insect species, in which it induces reproductive alterations. Phylogenetic analysis of Wolbachia strain of T. japonensis based on the sequences of 16S rDNA and ftsZ gene show agreement, indicating that this bacterium belongs to A-subdivision of Wolbachia.

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