지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Two potato (Solanum tuberosum L.) cultivars were transformed by Agrobacterium tumefaciens harboring cauliflower mosaic virus (CaMV) 35S promoter and $\beta$-glucuronidase (GUS) gene. Expression patterns of the CaMV 35S promoter according to tissue types and developmental stages, and genetic stability of GUS gene were investigated in the clonal progenies of transgenic potatoes. Kanamycin-resistant shoot emerged from tuber disc after 4 weeks of culture, and root was induced 6 weeks after culture on the selection medium. Shooting frequency of cvs. Superior and Dejima were 43% and 27%, respectively. Mature transformants and their clonal progenies showed no phenotypical abnormality. GUS activity was expressed primarily at parenchymatous cells of phloem tissue around the vascular cambium in the stem and root, and higher activity was found at the apical meristem of shoot, root and adventious shoot bud. GUS activity was higher at tubers of young explants than at stored tubers. These facts indicate that expression level of the CaMV 35S promoter differed according to tissue types and developmental stages of the organs. The GUS gene was stably inherited to each clonal progeny and normally expressed.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of a Screening System for Plant Defense-Inducing Agent using Transgenic Tobacco Plant with PR-la Promoter and GUS Gene
The Plant Pathology Journal
2005 .09
-
벼 종자 유래 배에서 외래유전자의 도입과 발현
식물학회지
1994 .01
-
Tissue Specific Expression of Wound-Inducible RCaM-2 Promoter in Transgenic Tobacco Plants
생명과학회지
2005 .04
-
Translational Increase of GUS Protein in Tobacco(Nicotiana tobacum L.) Transformed with Maize Intron Sequences and CaMV 35S Promoter
한국동물학회 학술대회
1994 .01
-
Improved in vitro Regeneration of Potato (Solanum tuberosum cv. Superior) Transformed by Agrobacterium Expressing $\beta-Glucuronidase$
식물학회지
1996 .01
-
Transformation System of Rice suspension-Cultured Microcolonies by Electroporation
식물학회지
1998 .01
-
Characterization of an Apple Anthocyanidin Synthase Gene in Transgenic Tobacco Plants
식물학회지
2006 .01
-
Cloning and Expression of β-Glucuronidase from Lactobacillus brevis in E. coli and Application in Bioconversion of Baicalin and Wogonoside
Journal of Microbiology and Biotechnology
2009 .01
-
A Novel Bi-directional Promoter Cloned from Melon and Its Activity in Cucumber and Tobacco
식물학회지
2008 .01
-
The increased GUS gene inactivation over generation in Arabidopsis transgenic lines
생명과학회지
2002 .02
-
Protocorm-like body를 이용한 호접란 형질전환 연구
생명과학회지
2009 .03
-
Introduction and Expression of Foreign Genes in Rice Cells by Particle Bombardment
식물학회지
1994 .01
-
Structural Characteristics of Two Wheat Histone H2A Genes Encoding Distinct Types of Variants and Functional Differences in Their Promoter Activity
한국식물학회 심포지움
1996 .01
-
Analysis of the Tissue-Specific S RNase Gene Promoter Associated with Self-Incompatibility in Tomato (Lycopersicon peruvianum L)
식물학회지
1999 .01
-
The First Intron of Petunia Actin-Depolymerizing Factor Gene Enhances GUS Expression in Transgenic Arabidopsis
한국동물학회 학술대회
2001 .01
-
Transcription of Human Interleukin-2 Gene in Nicotiana tabacum Driven by CaMV35S Promoter
식물학회지
1997 .01
-
Expression and Regulation of the RSI-1 Gene during Lateral Root Initiation
식물학회지
1999 .01
-
A Sterol Biosynthetic Gene AtCYP51A2 Promoter for Constitutive and Ectopic Expression of a Transgene in Plants
식물학회지
2008 .01
-
Characterization of Transgenic Potato (Solanum tuberosum L.) tubers with Sink MEtabolism-related gene
한국동물학회 학술대회
2001 .01
-
Expressional Characterization of Dehydroascorbate Reductase cDNA in Transgenic Potato Plants
식물학회지
2008 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0