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논문 기본 정보

자료유형
학술저널
저자정보
Hur, Moon-Hye (College of Pharmacy, Kyungsung University) Kang, Hee-Jin (College of Pharmacy, Kyungsung University) Min, Hye-Young (College of Pharmacy, Kyungsung University) Lee, Ji-Yeun (College of Pharmacy, Kyungsung University) Lee, Ki-Hyun (College of Pharmacy, Kyungsung University) Ahn, Moon-Kyu (College of Pharmacy, Kyungsung University)
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제20권 제1호
발행연도
1997.1
수록면
68 - 71 (4page)

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Penicillin sensor was prepared by immobilizing penicillinase (Pcase) on $H^{+}$-selective carboxylated poly (vinyl chloride) (PVC-COOH) membrane or cellulose filter membrane. The immobilization techniques are as follows. Pcase was immobilized with GTH on $H^{+}$-selective PVC-COOH membrane or some amount of BSA was dropped on that membrane. Another method to make immobilization is to mix type I Pcase with GTH and drop on a cellulose filter membrane. According to immobilization techniques, there were some differences in response properties of enzyme electrodes, however, all electrodes responded to Pcase-resistant penicillin derivatives. Pcase immobilized on cellulose filter membrane with $H^{+}$-selective PVC membrane eletrode was more stable and more sensitive to penicillinase-resistant penicillin derivatives than any other immobilization techniques.

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