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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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We elucidated the relationship between cell proliferation and somatic embryogenesis in the culture of carrot cotyledons. Fresh weights of the cotyledon explants were determined every five days while being cultured on a medium containing 2,4-D. Callus produrtion increased exponentially from Day 20 to Day 25, showing a two-fold rate of proliferation. Tn examine the embryogenic potential of the callus, we pre-cultured cotyledon explants on an MS medium with 2,4-D, then transferred them to an MS basal medium at five-day intervals. Somatic embryos formed most frequently when the cotyledons were pre-cultured for 20 days on an MS medium that contained 5 $\mu$M 2,4-D. The frequency of somatic embryo formation was 81%, while that of normal embryos with two cotyledons was 51% among those formed on a hormone-free medium. We used FACScan analysis to relate the embryogenic potential of the callus to the S phase in the cell cycle of cultured cells. The S phase was high after 25 days of culture on the medium with 5 $\mu$M 2,4-D. In contrast, the frequency of normal embryogenesis was higher at Day 20 of the pre-culture period. Culturing embryogenic calli on a medium with 5 $\mu$M 2,4-D was most favorable for producing somatic embryos with two cotyledons. We verified that active somatic embryogenesis was apparently related to tell division activity; somatic embryos induced from actively dividing tells were apt to accompany cotyledonary abnormality,
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Development of Abnormal Cotyledons on Carrot Somatic Embryos by Disturbed Auxin Distribution
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Effect of Humidity on Somatic Embryogenesis in Cotyledon Explant Culture of Carrot
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Origin of Somatic Embryo Induced from Cotyledons of Zygotic Embryos at Various Developmental Stages of Ginseng
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Factors Important for Somatic Embryogenesis in Zygotic Embryo Ewplant of Capsicum annuum L.
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Effect of Ammonium lon on Morphogenesis from Cultured Cotyledon Explants of Panax ginseng
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Turnover of Cell-Wall Polysaccharides during Somatic Embryogenesis and Development of Celery (Apium graveolens L.)
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Somatic Embryogenesis and Plantlet Formation from a Rare and Endangered Tree Species, Oplopanax elatus
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Improved Germination of Carrot Somatic Embryos by Disturbance of Auxin Action or Transport
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벼 진탕 배 배양세포로부터 원형질체 분리 및 배양
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세포조직배양계에서 재생된 식물의 발생 및 형태학적 다양성
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Effects of ABA on Secondary Embryogenesis from Somatic Embryos Induced from Inflorescence Culture of Aralia cordata Thunb
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Comparative Anatomy of Embryogenic and Non-Embryogenic Calli from Pimpinella brachycarpa
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2007 .01
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밀(Triticum aestivum L.)의 단세포 배양에 의한 식물체의 재분화
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1989 .01
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난과식물의 형질전환 유도 및 다량증식에 관한 연구 I. 자란 (Bletilla striata)의 미성숙 종자로부터 체세포배 형성 및 식물체 재분화
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