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논문 기본 정보

자료유형
학술저널
저자정보
Park Jong-Sug (Research Planning and Information Division, National Institute of Agricultural Biotechnology, RDA) Kim In-Sun (Biology Department, Keimyung University) Cho Moon-Soo (Division of Horticulture and Landscape Architecture, Daegu University) Park Shin (Division of Life and Environmental Science, Daegu University) Park Sang Gyu (Division of Life and Environmental Science, Daegu University)
저널정보
한국식물학회 식물학회지 식물학회지 제49권 제2호
발행연도
2006.1
수록면
133 - 140 (8page)

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Carrot seeds normally have surface spines. The availability of a spineless mutant would be agronomically beneficial, eliminating the current efforts to remove those spines pre-sowing. Furthermore, the identification of spine-specific genes would provide insights into spine development in wild-type carrot seed. This effort could be facilitated through the use of an annealing control primer (ACP) system. Here, we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) that involves ACPs for identifying genes of interest. With these techniques, 11 expressed sequence tags (ESTs) were obtained for cloning and sequencing the genes that are differentially expressed in wild-type spiny seeds, but not in the spineless mutant. In all, 7 cDNAs exhibited significant sequence similarity with known genes from other species. These included cell wall-associated hydrolase, tail fiber assembly protein, transcriptional regulatory protein, berberine bridge enzyme, S-adenosyl methionine synthase, transketolase, and phenylalanyl t-RNA synthetase beta chain. Four other cDNA sequences had no significant identities with known genes. As revealed by RT-PCR, these genes regulate spine formation during the developmental stage. Our results suggest that PCR-based differential display RT-PCR techniques are a very useful tool for identifying spine-specific genes from carrot seeds.

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