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자료유형
학술저널
저자정보
Park, Doo-Sang (Insect Resources Lab., Korea Research Insititute of Bioscience and Biotechnology) Shin, Sang-Woon (Insect Resources Lab., Korea Research Insititute of Bioscience and Biotechnology) Kim, Jong-Guk (Department of Microbiology, Kyung-pook National University) Park, Ho-Yong (Insect Resources Lab., Korea Research Insititute of Bioscience and Biotechnology)
저널정보
한국응용곤충학회 Journal of Asia-Pacific Entomology Journal of asia-pacific entomology 제4권 제1호
발행연도
2001.1
수록면
59 - 66 (8page)

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We previously identified a novel immunerelated serine type protease inhibitor cDNA from Hyphantria cuneas (Drury) (Shin et. al., 1998) that suggested a specific inhibitor directed at the protease (proPO) (Park et al., 200). Herein, we report complete nucleotide sequence and the exon-intron structure of the gene. The Hyphantria serpin gene spans 18kb and is composed of eight oxons and seven introns coding for an 1.8-kb mRNA. In the 5\` flanking sequence, we found several putative regulatory motifs including an enhancer, two NF-IF6 motifs, a TNF-$\alpha$ responsive element, an interferon-${\gamma}$-responsive element, and a GATA motif element that have been found in the promoters of the acute phase protein genes of mammals and insects. In addtiion, a 9-bp inverted repeat sequence located from -270 to -221 region was also found. A reactive site loop sequence was located in exon 8 flanked by 3' untranslation sequence. Phylogenetic analysis showed that this Hyphantria serpin did not cluster with either insect or mammalian serpins that are known, or suspected, to be associated with immune function. Southern blot analysis showed that the Hyphantria serpin gene is present as a single copy in the genome.

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