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자료유형
학술저널
저자정보
Han, Shin-Ha (Department of Pharmacy, Sahmyook University) Sung, Ki-Hyun (Department of Pharmacy, Sahmyook University) Yim, Dong-Sool (Department of Pharmacy, Sahmyook University) Lee, Sook-Yeon (Department of Pharmacy, Sahmyook University) Cho, Kyung-Hae (Department of Biology, Seoul Womens University) Lee, Chong-Kil (College of Pharmacy, Chungbuk National University) Ha, Nam-Joo (Department of Pharmacy, Sahmyook University) Kim, Kyung-Jae (Department of Pharmacy, Sahmyook University)
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제25권 제6호
발행연도
2002.1
수록면
895 - 902 (8page)

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Monocytes and macrophages playa major role in defense mechanism of the host response to tumor, in part through the secretion of several potent products and macrophage cytokines. Monocytes and tissue macro phages produce at least two groups of protein mediators of inflammation, interleukin 1 (IL-1) and tumor necrosis factor (TNF). Recent studies emphasizes that TNF and IL-1 modulate the inflammatory function of endothelial cells, leukocytes, and fibroblasts. In this study, our work is directed toward studying the in vitro effects of Korean propolis on the ability to induce cellular and secretory responses in murine macrophage cell line, RAW 264.7. It was found that Water Extract of Korean Propolis (WEP) could activate macro phages by producing cytokines. The production of the macrophage cytokines, IL-1 and TNF-$\alpha$, by RAW 264.7 treated with WEP was examined from 2.5 $\mu\textrm{g}$/ml up to 25 $\mu\textrm{g}$/ml with dose dependent manner. Nitric oxide (NO) production was also increased when cells were exposed to combination of LPS and WEP from 2.5 $\mu\textrm{g}$/ml up to 25 $\mu\textrm{g}$/ml. At high dose of WEP (50 to 100 $\mu\textrm{g}$/ml) used to prescribe for anti-inflammatory and analgesic medicine showed inhibition of NO production in LPS-stimulated macrophage. Besides cytokine production, NO release, surface molecule expression and cell morphologic antigen expression were increased in response to the stimulation by WEP. These results suggested WEP may function through macrophage activation.

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