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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 99 - 107 (9page)
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Objectives : The aim of this study was to authenticate whether fractionated extract of Rumex japonicus HOUTT. (RJ) has anti-inflammatory effects in mouse macrophage, RAW264.7 cells. Methods : Roots of RJ were extracted by methanol for 48hours. The methanol that gained was filtered and freeze dried. The methanol extract was dissolved in water and dichloromethane (DCM). After that, two layers were separated. Ethyl acetate (EA) added to the water layer and separated again. All the layers were filtered and freeze dried and the extracts were tested. Cytotoxic activity of extracts on RAW 264.7 cells was measured using MTS assay. The nitric oxide (NO) production was measured and proinflammatory cytokines and $PGE_2$ were measured by ELISA kit. The levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), I ${\kappa}$-B-${\alpha}$ and nuclear NF-${\kappa}B$ p65 expression were detected by western blot. Results : Our results indicated that DCM and EA extracts of RJ inhibited the LPS-induced NO, $PGE_2$ production and iNOS, COX-2 expression accompanied by an attenuation of TNF-${\alpha}$, IL-$1{\beta}$ and IL-6 production in RAW 264.7 cells most effectively. DCM and EA extracts also had suppression effects of LPS-induced NF-${\kappa}B$ and MAPKs activation. Conclusions : This results demonstrate that fractionated extract of RJ has anti-inflammatory effects and among the fractioned extract, dichloromethane and ethyl acetate extract have best anti-inflammatory effects.
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참고문헌 (29)
참고문헌 신청
Hernández-Ledesma B. / 2009 / Antioxidant and anti-inflammatory properties of cancer preventive peptide lunasin in RAW 264. 7 macrophages / Biochem Biophys Res Commun 390(3):803~808
College of Korean medicine, Professors ofHerbology, collaborator / 2000 / Herbology / Younglimsa :398~399
장대식 / 200512 / 24-nor-ursane type triterpenoids from the stems of Rumex japonicus / Chemical and Pharmaceutical Bulletin 53(12):1594~1596
Jiang L. / 2007 / Oxanthrone C-glycosides and epoxynaphthoquinol from the roots of Rumex japonicus / Phytochemistry 68(19):2444~2449
Chen M. / 2009 / A new anthraquinone from roots of Rumex japonicus / Zhongguo Zhong Yao Za Zhi 34(17):2194~2196
College of Korean medicine, Professors ofHerbology, collaborator / 2000 / Herbology / Younglimsa :398~399
장대식 / 200512 / 24-nor-ursane type triterpenoids from the stems of Rumex japonicus / Chemical and Pharmaceutical Bulletin 53(12):1594~1596
Jiang L. / 2007 / Oxanthrone C-glycosides and epoxynaphthoquinol from the roots of Rumex japonicus / Phytochemistry 68(19):2444~2449
Chen M. / 2009 / A new anthraquinone from roots of Rumex japonicus / Zhongguo Zhong Yao Za Zhi 34(17):2194~2196
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