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학술저널
저자정보
Lee, Sang Won (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) Lee, Yu Geon (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) Cho, Jeong-Yong (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) Kim, Young Chool (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) Lee, Sang-Hyun (Korea Pear Research Organization, Chonnam National University) Kim, Wol-Soo (Korea Pear Research Organization, Chonnam National University) Moon, Jae-Hak (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제58권 제3호
발행연도
2015.1
수록면
335 - 341 (7page)

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Chlorogenic acid [3-(3,4-dihydroxycinnamoyl) quinic acid, CGA] is one of the main compounds contained in immature pear fruits. In the present study, we established simple purification of CGA in high purity from the immature fruit of pear (Pyrus pyrifolia Nakai cv. Chuhwangbae) which contains CGA in high concentration. The 80 % MeOH extract (1.4 g) of immature pear fruits (20 g fresh wt.) was partitioned with ethyl acetate (EtOAc) under acidic condition (pH 2.65). Then, the EtOAc-soluble acidic layer (110.3 mg) was further purified using Diaion HP-20 column chromatography ($H_2O/MeOH$, step wise). Then, the CGA-contained fractions were finally purified by preparative ODS-HPLC. The isolated CGA (24.3 mg) showed high purity (>99 %) and the total recovery was estimated to be 63.9 %.

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