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학술저널
저자정보
Wang, Chenxi (School of Life Science, Jiangxi Science & Technology Normal University) Li, Yuping (School of Life Science, Jiangxi Science & Technology Normal University) Yao, Lihua (School of Life Science, Jiangxi Science & Technology Normal University) Wu, Guangjie (School of Life Science, Jiangxi Science & Technology Normal University) Chang, Jun (School of Life Science, Jiangxi Science & Technology Normal University) Shu, Chengchuang (School of Life Science, Jiangxi Science & Technology Normal University) Chen, Meiqin (School of Life Science, Jiangxi Science & Technology Normal University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제57권 제5호
발행연도
2014.1
수록면
647 - 653 (7page)

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Optimization of ultrasonic extraction of Portulaca oleracea L. flavonoids (POF) was investigated using single-factor experimentation combined with response surface methodology. The optimal conditions for the highest yield (16.25 mg RE/g DW) of POF was 39.01% ethanol, $55.25^{\circ}C$ extraction temperature, 15 min extraction time, and 23.92 (v/m) liquid-to-solid ratio. The crude extract of POF was purified on the polyamide resin. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole assays of the flavonoids were evaluated, which suggested the concentrations of the flavonoids (0-1 mg/mL) and quercetin (0-1 mg/mL). Quercetin was identified in the extract by comparing relative retention time of the reference standard.

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