지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 406 - 420 (15page)
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초록· 키워드
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Objectives : Scutellaria barbata has been widely used in oriental medicine used for treatment of acute and chronic inflammatory diseases. In this study, to investigate the protective effect of Scutellaria barbata on type I allergic response, we determined whether Scutellaria barbata inhibits early or late allergic responses. Methods : To assess the effect of Scutellaria barbata Pharmacopuncture Extract(SB) in RBL-2H3 cells, we investigated the levels of the markers of degranulation such as ${\beta}$-hexosaminidase and histamine, inflammatory mediator such as IL-4, TNF-${\alpha}$, PGE2 and cysLT, and mRNA expression of cytokines and enzymes. In addition, we determined the levels of intracellular ROS by DCFH-DA assay and the free radical scavenging activity by DPPH method. Results : We found that SB suppressed the release of ${\beta}$-hexosaminidase and histamine and the production of IL-4, TNF-${\alpha}$, PGE2 and cysLT in RBL-2H3 by the antigen stimulation. SB also significantly inhibited the enzyme mRNA expressions, such as HDC2, COX-1, COX-2, 5-LOX and iNOS2, along with reduced cytokine mRNA expressions, such as IL-$1{\beta}$, IL-2, IL-3, IL-4, IL-5, IL-6, IL-13, TNF-${\alpha}$ and GM-CSF in RBL-2H3. In addition, SB suppressed the levels of intracellular ROS. Conclusions : Our results indicate that SB protects against type I allergic response and exert an anti-inflammatory effect through the inhibition of degranulation, inflammatory mediator release and mRNA expression of cytokines and enzymes.
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등록된 정보가 없습니다.
참고문헌 (37)
참고문헌 신청
Forstermann U / 1995 / Nitric oxide synthase: expression and expressional control of the three isoforms / Naunyn-schmiedeberg's Archives of Pharmacology 352(4):351~364
Matsui T / 2000 / Diphenyleneiodonium prevents reactive oxygen species generation, tyrosine phosphorylation, and histamine release in RBL-2H3 mast cells / Biochemical and BiophySical Research Communications 276(2):742~748
Swindle EJ / 2007 / FceRI- and Fc$\gamma$ receptormediated production of reactive oxygen species by mast cells is lipoxygenase- and cyclooxygenase- dependent and NADPH oxidaseindependent / Journal of Immunology 179(10):7059~7071
Guo SS / 2009 / The cytology mechanism of anti-parainfluenza virus infection of total flavone of Scutellaria barbata / Yao Xue Xue Bao Acta Pharmaceutica Sinica 44(12):1348~1352
Sato Y / 2000 / Phytochemical flavones isolated from Scutellaria barbata and antibacterial activity against methicillin- resistant Staphylococcus aureus / J Ethnopharmacol 72(3):483~488
Matsui T / 2000 / Diphenyleneiodonium prevents reactive oxygen species generation, tyrosine phosphorylation, and histamine release in RBL-2H3 mast cells / Biochemical and BiophySical Research Communications 276(2):742~748
Swindle EJ / 2007 / FceRI- and Fc$\gamma$ receptormediated production of reactive oxygen species by mast cells is lipoxygenase- and cyclooxygenase- dependent and NADPH oxidaseindependent / Journal of Immunology 179(10):7059~7071
Guo SS / 2009 / The cytology mechanism of anti-parainfluenza virus infection of total flavone of Scutellaria barbata / Yao Xue Xue Bao Acta Pharmaceutica Sinica 44(12):1348~1352
Sato Y / 2000 / Phytochemical flavones isolated from Scutellaria barbata and antibacterial activity against methicillin- resistant Staphylococcus aureus / J Ethnopharmacol 72(3):483~488
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