지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Adipocytes have been known to secrete a number of important proteins called adipokines with roles in energy metabolism, reproduction, cardiovascular function and immunity. In this study we have attempted to identify intensively secretory proteins from 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into mature adipocytes and then the cells were left in serum-free medium. The supernatant was filtrated and dialyzed. Lyophilized secretome was fractionated by two different methods, 1-D SDS PAGE and RP-FPLC. The tryptic peptides from the gel slices and the FPLC fractions were analyzed by nanoLC/ESI-MS/MS. We identified a total of 303 identical proteins from two methods, 251 proteins from 1-D gel and 184 proteins from RP-FPLC. 86 of them were listed as a secretory protein Finally, we identified many known or unknown secreted proteins existed in the low level including adiponectin, angiotensinogen, bone morphogenetic protein-1 (BMP-1), macrophage migration inhibitory factor (MIF), insulin like growth factor-II (IGF-II), interleukin-6 (IL-6), follistatin-related protein-1, minecan, and resistin. The existence of some of secreted proteins has been confirmed in RNA level. This proteomic experiment is useful for the intensive screening of secretory proteins in many kinds of other cells.
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참고문헌 (31)
참고문헌 신청
Keaney, J. F / 2003 / Obesity and systemic oxidative stress: clinical correlates of oxidative stress in the Framingham Study / Arterioscler. Thromb. Vasc. Biol 23:434
Gastaldelli, A. / 2002 / Metabolic effects of visceral fat accumulation in type 2 diabetes / J. Clin. Endocrinol. Metab 87:5098
Cook, K. S / 1987 / Adipsin: a circulating serine protease homolog secreted by adipose tissue and sciatic nerve / Science 237:402
Flier, J. S. / 1987 / Severely impaired adipsin expression in genetic and acquired obesity / Science 237:405
Scherer, P. E / 1995 / A novel serum protein similar to C1q, produced exclusively in adipocytes / J. Biol. Chem 270:26746
Gastaldelli, A. / 2002 / Metabolic effects of visceral fat accumulation in type 2 diabetes / J. Clin. Endocrinol. Metab 87:5098
Cook, K. S / 1987 / Adipsin: a circulating serine protease homolog secreted by adipose tissue and sciatic nerve / Science 237:402
Flier, J. S. / 1987 / Severely impaired adipsin expression in genetic and acquired obesity / Science 237:405
Scherer, P. E / 1995 / A novel serum protein similar to C1q, produced exclusively in adipocytes / J. Biol. Chem 270:26746
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