지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제37권 제5호
- 발행연도
- 2014.1
- 수록면
- 636 - 644 (9page)
이용수
초록· 키워드
오류제보하기
A liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and applied for the determination of human $A{\beta}1$-40 and $A{\beta}1$-42 peptides in transgenic mouse plasma to support preclinical pharmacodynamics studies. The method consisted of micro-elution solid phase extraction for sample preparation and LC-MS/MS analysis in the negative ion mode using electrospray ionization for analysis. $^{15}N_{53}-A{\beta}1$-40 and $^{15}N_{55}-A{\beta}1$-42 peptides were used as internal standards. A quadratic regression (weighted 1/concentrations), with an equation $y=ax^2+bx+c$, was used to fit calibration curves over the concentration range of 0.500-100 ng/mL for both $A{\beta}1$-40 and $A{\beta}1$-42 peptides. For quality control samples at 6.00, 40.0 and 80.0 ng/mL from the qualification experiment, the within-run accuracy ranged from -2.69 to 0.583 % with precision values ${\geq}8.23%$ for $A{\beta}1$-40. Within-run accuracy ranged from -4.83 to 10.1 % with precision values ${\geq}8.87%$ for $A{\beta}1$-42. Samples from a pharmacodynamics study using Tg2576 transgenic mice were analyzed by this qualified LC-MS/MS method and concentrations were compared to those generated by ELISA. The two methods were shown to be comparable for $A{\beta}1$-40 quantification of samples from the Tg2576 amyloid precursor protein transgenic mouse model, but varied slightly for $A{\beta}1$-42.
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