지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to detect and identify four fish viruses, fish iridovirus, viral hemorrhagic septicaemia virus (VHSV), viral nervous necrosis virus (VNNV), hirame rhabdovirus (HRV). Four viruses were detected by PCR with each specific primers. Identification of iridovirus was achieved by digesting the PCR amplified fragment with a restriction enzyme ApaⅠ. It was possible to distinguish positive from false positive PCR amplicons of VHSV by RFLP of PstⅠ or HindⅢ restriction enzymes. VNNV was identified using RFLP of BamHⅠrestriction enzyme and HRV was identified by XbaⅠ restriction enzyme. This approach can be used for more rapid, simple and specific diagnosis of fish viral diseases.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Cutrin, J. M. / 2004 / Restriction fragment length polymorphisms and sequence analysis : an approch for genotyping infectious pancreatic necrosis virus reference strains and other aquabirnaviruses isolated frpom northwestern Spain / Appl. Environ. Microbiol 70:1059~1067
Dubach, M.M. / 2001 / Rapid Discrimination among Dermatophytes, Scytalidium spp., and Other Fungi with a PCR-Restriction Fragment Length Polymorphism Ribotyping Method / J Clin Microbiol 39:685~690
DO, J. W. / 2005 / Sequence variation in the gene encoding the major capsid protein of korean fish iridoviruses / Archives of Virology 150:351~359
Gilad, O. / 2003 / Molecular comparison of isolates of an emerging fish pathogen, koi herpesvirus, and the effect of water temperature on mortality of experimentally infected koi / J. Gen. Virol 84:2661~2667
Gorie, S. / 1985 / Disease of cultured hirame (Japanese flounder) Paralichtys olovaceus I. Preliminary report on a disease of marine pen cultured flounder may be caused by viral infection / Bull. Hyogo. Pref. Fish Exp. Stn :66~68
Dubach, M.M. / 2001 / Rapid Discrimination among Dermatophytes, Scytalidium spp., and Other Fungi with a PCR-Restriction Fragment Length Polymorphism Ribotyping Method / J Clin Microbiol 39:685~690
DO, J. W. / 2005 / Sequence variation in the gene encoding the major capsid protein of korean fish iridoviruses / Archives of Virology 150:351~359
Gilad, O. / 2003 / Molecular comparison of isolates of an emerging fish pathogen, koi herpesvirus, and the effect of water temperature on mortality of experimentally infected koi / J. Gen. Virol 84:2661~2667
Gorie, S. / 1985 / Disease of cultured hirame (Japanese flounder) Paralichtys olovaceus I. Preliminary report on a disease of marine pen cultured flounder may be caused by viral infection / Bull. Hyogo. Pref. Fish Exp. Stn :66~68
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