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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 327 - 343 (17page)
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초록· 키워드
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In order to develop a high cellulolytic direct-fed microorganism (DFM) for ruminant productivity improvement, this study isolated cellulolytic bacteria from the rumen of Holstein dairy cows, and compared their cellulolytic abilities via DM degradability, gas production and cellulolytic enzyme activities. Twenty six bacteria were isolated from colonies grown in Dehority's artificial (DA) medium with 2% agar and cultured in DA medium containing filter paper at $39^{\circ}C$ for 24h. 16s rDNA gene sequencing of four strains from isolated bacteria showed that H8, H20 and H25 strains identified as Ruminococcus flavefaciens, and H23 strain identified as Fibrobacter succinogenes. H20 strain had higher degradability of filter paper compared with others during the incubation. H8 (R. flavefaciens), H20 (R. flavefaciens), H23 (F. succinogenes), H25 (R. flavefaciens) and RF (R. flavefaciens sijpesteijn, ATCC 19208) were cultured in DA medium with filter paper as a single carbon source for 0, 1, 2, 3, 4 and 6 days without shaking at $39^{\circ}C$, respectively. Dry matter degradability rates of H20, H23 and H25 were relatively higher than those of H8 and RF since 2 d incubation. The cumulative gas production of isolated cellulolytic bacteria increased with incubation time. At every incubation time, the gas production was highest in H20 strain. The activities of carboxymethylcellulase (CMCase) and Avicelase in the culture supernatant were significantly higher in H20 strain compared with others at every incubation time (p<0.05). Therefore, although further researches are required, the present results suggest that H20 strain could be a candidate of DFM in animal feed due to high cellulolytic ability.
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참고문헌 (33)
참고문헌 신청
Beharka, A. A. / 1993 / Effect of Aspergillus oryzae fermentation extract (Amaferm®) on in vitro fiber degradation / J. Dairy Sci 76:812~818
Bryant, M. P. / 1973 / Nutritional requirements of predominant rumen cellulolytic bacteria / Fed. Proc 32:1809
Bryant, M. P. / 1953 / Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen / J. Dairy Sci 36:205~217
Bryant, M. P. / 1953 / Numbers and some predominant groups of bacteria in the rumen of cows fed different rations / J. Dairy Sci 36:218~224
Beuvink, J. M. W. / 1992 / Interactions between substrate, fermentation end-products, buffering systems and gas production upon fermentation of different carbohydrates by mixed rumen microorganisms in vitro / Appl. Microbiol. Biotechnol 37:505~509
Bryant, M. P. / 1973 / Nutritional requirements of predominant rumen cellulolytic bacteria / Fed. Proc 32:1809
Bryant, M. P. / 1953 / Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen / J. Dairy Sci 36:205~217
Bryant, M. P. / 1953 / Numbers and some predominant groups of bacteria in the rumen of cows fed different rations / J. Dairy Sci 36:218~224
Beuvink, J. M. W. / 1992 / Interactions between substrate, fermentation end-products, buffering systems and gas production upon fermentation of different carbohydrates by mixed rumen microorganisms in vitro / Appl. Microbiol. Biotechnol 37:505~509
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