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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 49 - 54 (6page)
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Objective : We recently reported that OMC-2010 has an immuno-modulatory effects via inhibiting tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-5. However, we did not find out which constituents play an important role in immuno-modulatory effect of OMC-2010. Thus, this study was performed to estimate the effects of constituents of OMC-2010 on cytokine production in mouse spleen cells, then ultimately reach to find out effective constituents regulating splenic cytokine production. Methods : Mouse spleen cells were pre-treated with water and ethanol extract of constituents of OMC-2010 such as Rehmannia glutinosa (RG), Pinellia ternata (PT), Citrus unshiu Markovich (CUM), Glycyrrhiza uralensis (GU), Platycodon grandiflorum (PG), Schisandra chinensis (SC). After 1 h, the cells were stimulated with lipopolysaccharide (LPS, 1 ${\mu}g/ml$) for 48 h. Then the cells were harvested for real-time reverse transcription polymerase chain reaction to detect cytokine productions. Results : The water extract of RG extract significantly inhibited the LPS-induced inTNF-${\alpha}$ and IL-5 mRNA expressions, but the water extract of PT, CUM, GU, PG, and SC did not. The ethanol extract of RG, PT, and SC significantly inhibited the LPS-induced TNF-${\alpha}$, and IL-5 mRNA expressions, but the ethanol extract of CUM, GU, and PG did not. Conclusions : Theses results could suggest that the water extract of RG and the ethanol extract of RG, PT, and SC inhibited the expression of TNF-${\alpha}$ and IL-5, which means that the possible candidate of OMC-2010 water extract's action might be RG, and ethanol extract's action might be RG, PR, and SC.
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참고문헌 (16)
참고문헌 신청
Lichtor T. / 2012 / Immunogene therapy / Adv Exp Med Biol 746:151~165
Del Portillo HA. / 2012 / The role of the spleen in malaria / Cell Microbiol 14(3):343~355
Tan JK. / 2010 / In vitro haematopoiesis of a novel dendritic-like cell present in murine spleen / Curr Stem Cell Res Ther 5(4):367~371
Van den Eertwegh AJ. / 1992 / Immunological functions and in vivo cell-cell interactions of T cells in the spleen / Crit Rev Immunol 11(6):337~380
De Sousa M. / 1992 / T lymphocytes and iron overload: novel correlations of possible significance to the biology of the immunological system / Mem Inst Oswaldo Cruz. 87(Suppl 5):23~29
Del Portillo HA. / 2012 / The role of the spleen in malaria / Cell Microbiol 14(3):343~355
Tan JK. / 2010 / In vitro haematopoiesis of a novel dendritic-like cell present in murine spleen / Curr Stem Cell Res Ther 5(4):367~371
Van den Eertwegh AJ. / 1992 / Immunological functions and in vivo cell-cell interactions of T cells in the spleen / Crit Rev Immunol 11(6):337~380
De Sousa M. / 1992 / T lymphocytes and iron overload: novel correlations of possible significance to the biology of the immunological system / Mem Inst Oswaldo Cruz. 87(Suppl 5):23~29
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