지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 39 - 44 (6page)
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초록· 키워드
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Objectives : The purpose of this study was to identify active fraction and components from antiplatelet Ulmi cortex extract. Methods : The 70% ethanol extract of Ulmi cortex was subjected to column chromatography over D101 resin and eluted with an 20% (W1), 30% (W2), 40% (W3), 50%(W4), 70% (W5), and 100% ethanol (W6) to yield 6 fractions. W6 was further fractioned and its active components were purified using semi-preparative HPLC. The isolated compounds were identified by MS and NMR, and their contents were simultaneously analyzed using HPLC/UV. Antiplatelet aggregation activities of the fractions and the compounds were evaluated using rat platelet-rich plasma in presence of collagen ($5{\mu}g/ml$), arachidonic acid (0.05 U/ml), or thrombin ($100{\mu}M$). Results : Among six fractions, W3 prominently inhibited platelet aggregation. At the concentration of $200{\mu}g/ml$, W3 strongly inhibited arachidonic acid- and collagen-induced platelet aggregations by 78.2% and 65.9%, respectivley, and weakly inhibited thrombin-inducded platelet aggregation by 32.6%. Catechin, epicatehin, and catechin-7-O-${\beta}$-D-glucopyranoside were isolated from W3 and their contents were revealed to be 15.1%, 0.87%, and 0.32%. Catechin and epicatechin at the concentrations of $100{\mu}M$ strongly inhibited collagen-induced platelet aggregation by 79.9% and 86.6%, respectively, but weakly inhibited arachidonic acid- and thrombin-induced platelet aggregations. Conclusions : A main active principle of anitplatelet Ulmi Cortex extract is W3 fraction, of which main active component is catechin considering its antiplatelet activity and content.
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참고문헌 (14)
참고문헌 신청
Kim YW / 1999 / Evaluation of thrombolytic effect of streptokinase-dextran conjugate in a rat model of arterial hrombosis / Korean J Pharm Sci 29:211~216
Yun YP / 1996 / The antithrombotic effect effect of green tea catechins / Korean J Food Hyg Safety 11:77~82
Lee HS / 1995 / How safe is the readministration of streptokinase? / Drug Saf 13:76~80
Halliwell B / 1999 / Establishing the significance and optimal intake of dietary antioxidants: The biomarker concept / Nutr Rev 57:104~113
Yang GH / 1982 / Toxicological studies of antioxidants, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) / Korean J Food Sci Technol 14:283~288
Yun YP / 1996 / The antithrombotic effect effect of green tea catechins / Korean J Food Hyg Safety 11:77~82
Lee HS / 1995 / How safe is the readministration of streptokinase? / Drug Saf 13:76~80
Halliwell B / 1999 / Establishing the significance and optimal intake of dietary antioxidants: The biomarker concept / Nutr Rev 57:104~113
Yang GH / 1982 / Toxicological studies of antioxidants, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) / Korean J Food Sci Technol 14:283~288
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