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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 71 - 78 (8page)
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초록· 키워드
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Objectives : This study was fulfilled to investigate nominee materials as anti-inflammatory agent from ethanol extract of Chinese medicinal plants in Yanjin. Among the 20 candidates, we selected most effective one, the ethanol extract of Cicuta virosa L. (CVL). The mechanism underlying the anti-inflammatory effects of CVL is not clearly identified as yet. Accordingly, we clarified the anti-inflammatory effects of CVL and its underlying molecular mechanisms in LPS-stimulated RAW 264.7 macrophages. Methods : RAW264.7 macrophages were incubated with CVL (12.5, 25, or $50{\mu}M$) and/or lipopolysaccharide (LPS) ($1{\mu}g/m{\ell}$). Cytotoxicity was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the level of nitric oxide (NO) production was measured with Griess reagent. The prostaglandin $E_2$ ($PGE_2$) production was measured with enzyme immunoassay kits and the protein expression of inducible nitric oxide synthase (iNOS) was determined using Western blot analysis. Results : Among the 20 ethanol extract of Chinese medicinal plants of Yanjin tested, CVL significantly reduced the production of NO in a dose-dependent manner via inhibition the protein expressions of iNOS without cytotoxicity on the LPS-stimulated RAW 264.7 macrophages. In addition, CVL also effectively declined the production of $PGE_2$ in LPS-simulated RAW 264.7 macrophages. Conclusions : Taken together, these data presented in this study demonstrate that CVL possesses anti-inflammatory activity by suppressing the production of pro-inflammatory mediators NO and $PGE_2$, and pro-inflammatory protein iNOS expression in LPS-stimulated RAW 264.7 macrophages.
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참고문헌 (21)
참고문헌 신청
Choi, Y.H / 2014 / Anti-inflammatory effects of cordycepin in lipopolysaccharidestimulated RAW 264.7 macrophages through Tolllike receptor 4-mediated suppression of mitogenactivated protein kinases and NF-kappaB signaling pathways / Drug Des Devel Ther 8:1941~1953
Lee, W. / 2017 / Xylose-Taurine Reduced Suppresses the Inflammatory Responses in Lipopolysaccharide-Stimulated Raw264.7 Macrophages / Adv Exp Med Biol 975:633~642
Wang, P. / 2016 / Inhibitory effects of geraniin on LPSinduced inflammation via regulating NF-kappaB and Nrf2 pathways in RAW 264.7 cells / Chem Biol Interact 253:134~142
Oh, Y.C. / 2012 / Fermentation by Lactobacillus enhances anti-inflammatory effect of Oyaksungisan on LPS-stimulated RAW 264.7mouse macrophage cells / BMC Complement Altern Med 12:17
Lee, H.J / 2017 / Ethanol Extract of Potentilla supina Linne Suppresses LPS-induced Inflammatory Responses through NF-kappaB and AP-1 Inactivation in Macrophages and in Endotoxic mice / Phytother Res 31(3):475~487
Lee, W. / 2017 / Xylose-Taurine Reduced Suppresses the Inflammatory Responses in Lipopolysaccharide-Stimulated Raw264.7 Macrophages / Adv Exp Med Biol 975:633~642
Wang, P. / 2016 / Inhibitory effects of geraniin on LPSinduced inflammation via regulating NF-kappaB and Nrf2 pathways in RAW 264.7 cells / Chem Biol Interact 253:134~142
Oh, Y.C. / 2012 / Fermentation by Lactobacillus enhances anti-inflammatory effect of Oyaksungisan on LPS-stimulated RAW 264.7mouse macrophage cells / BMC Complement Altern Med 12:17
Lee, H.J / 2017 / Ethanol Extract of Potentilla supina Linne Suppresses LPS-induced Inflammatory Responses through NF-kappaB and AP-1 Inactivation in Macrophages and in Endotoxic mice / Phytother Res 31(3):475~487
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