Comparison of in Vitro Cytotoxicity and Apoptogenic Activity of Magnesium Chloride and Cisplatin as Conventional Chemotherapeutic Agents in the MCF-7 Cell Line

Mirmalek, Seyed Abbas; Jangholi, Ehsan; Jafari, Mohammad; Yadollah-Damavandi, Soheila; Javidi, Mohammad Amin; Parsa, Yekta; Parsa, Tina; Salimi-Tabatabaee, Seyed Alireza; Kolagar, Hossein Ghasemzadeh; Jalil, Saeed Khazaei; Alizadeh-Navaei, Reza

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자료유형: 학술저널

저자정보: Mirmalek, Seyed Abbas (Department of Surgery, Islamic Azad University, Tehran Medical Sciences Branch) Jangholi, Ehsan (Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch) Jafari, Mohammad (Department of Surgery, Faculty of Medical Sciences, Arak University of Medical Sciences) Yadollah-Damavandi, Soheila (Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch) Javidi, Mohammad Amin (Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University) Parsa, Yekta (Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch) Parsa, Tina (Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch) Salimi-Tabatabaee, Seyed Alireza (Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch) Kolagar, Hossein Ghasemzadeh (Students Research Committee, School of Public Health, Shahroud University of Medical Sciences) Jalil, Saeed Khazaei (School of Nursing and Midwifery, Shahroud University of Medical Sciences) Alizadeh-Navaei, Reza (Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제17권 제3호

발행연도: 2016.1

수록면: 131 - 134 (4page)

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Breast cancer is the most common malignancy and also the second leading cause of cancer death among women and also in women that have a high mortality. Previous studies showed that magnesium (Mg) has cytotoxic effects on malignant cell lines. However, the anti-cancer effects of Mg on MCF-7 breast cancer cells are uncertain. This study was aimed at the comparison of the cytotoxic effect of Mg salt (MgCl2) and cisplatin on MCF-7 cells and fibroblasts (as normal cells). After treatment with various concentrations of MgCl2, and cisplatin as a positive control for 24 and 48 hours (h), cytotoxicity activity was measured by MTT assay. In addition, apoptosis was determined by annexin V/propidium iide assay. Both cisplatin and the MgCl2 exhibited dose-dependent cytotoxic effects in the MCF-7 cell line, although the LD50 of the Mg was significantly higher when compared to cispaltin ($40{\mu}g/ml$ vs. $20{\mu}g/ml$). Regarding annexin V/propidium results, treatment of MCF-7 cells with LD50 concentrations of cisplatin and Mg showed 59% and 44% apoptosis at 24h, respectively. Finally, the results indicated that Mg has cytotoxic effects on MCF-7 cells, but less than cisplatin as a conventional chemotherapeutic agent. However, regarding the side effects of chemotherapy drugs, it seems that Mg can be considered as a supplement for the treatment of breast cancer.

#Breast cancer #MCF-7 cells #cisplatin #magnesium #apoptosis

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