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논문 기본 정보

자료유형
학술저널
저자정보
Lim, Kitaek (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) Park, Soo Hyun (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) Kim, Jangho (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) SeonWoo, Hoon (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) Choung, Pill-Hoon (Department of Oral and Maxillofacial Surgery and Dental Research Institute, School of Dentistry, Seoul National University) Chung, Jong Hoon (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
저널정보
한국농업기계학회 바이오시스템공학(구 한국농업기계학회지) 바이오시스템공학 제38권 제1호
발행연도
2013.1
수록면
55 - 63 (9page)

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Purpose: The present study aimed at image processing methods for automatic cell pattern recognition and morphological analysis for tissue engineering applications. The primary aim was to ascertain the novel algorithm of adaptive brightness correction from microscopic images for use as a potential image analysis. Methods: General microscopic image of cells has a minor problem which the central area is brighter than edge-area because of the light source. This may affect serious problems to threshold process for cell-number counting or cell pattern recognition. In order to compensate the problem, we processed to find the central point of brightness and give less weight-value as the distance to centroid. Results: The results presented that microscopic images through the brightness correction were performed clearer than those without brightness compensation. And the classification of mixed cells was performed as well, which is expected to be completed with pattern recognition later. Beside each detection ratio of hBMSCs and HeLa cells was 95% and 92%, respectively. Conclusions: Using this novel algorithm of adaptive brightness correction could control the easier approach to cell pattern recognition and counting cell numbers.

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