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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 382 - 388 (7page)
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초록· 키워드
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A duplex real-time polymerase chain reaction (PCR) based assay for the detection of porcine and horse meat in sausages was designed by using EvaGreen fluorescent dye. Primers were selected from mitochondrial 12S rRNA and 16S rRNA genes which are powerful regions for identification of horse and porcine meat. DNA from reference samples and industrial products was successfully extracted using the GIDAGEN® Multi-Fast DNA Isolation Kit. Genomes were identified based on their specific melting peaks (Mp) which are 82.5℃ and 78℃ for horse and porcine, respectively. The assay used in this study allowed the detection of as little as 0.0001% level of horse meat and 0.001% level of porcine meat in the experimental admixtures. These findings indicate that EvaGreen based duplex realtime PCR is a potentially sensitive, reliable, rapid and accurate assay for the detection of meat species adulterated with porcine and horse meats.
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등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Asensio, L. / 2008 / Determination of food authenticity by enzyme-linked immunosorbent assay (ELISA) / Food Cont 19:1~8
Cawthorn, D. M. / 2013 / A high incidence of species substitution and mislabelling detected in meat products sold in South Africa / Food Cont 32:440~449
Commission Directive 2001/101/EC of 26 November 2001amending Directive 2000/13/EC of the European Parliament and of the Council on the approximation of the laws of the Member States relating to the labelling, presentation and advertising of foodstuffs
Eischeid, A. C. / 2011 / SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR / BMC Res. Notes 4:263
El zaqu vel, P. / 2008 / A multiplex RT-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables / Food Microbiol 25:705~713
Cawthorn, D. M. / 2013 / A high incidence of species substitution and mislabelling detected in meat products sold in South Africa / Food Cont 32:440~449
Commission Directive 2001/101/EC of 26 November 2001amending Directive 2000/13/EC of the European Parliament and of the Council on the approximation of the laws of the Member States relating to the labelling, presentation and advertising of foodstuffs
Eischeid, A. C. / 2011 / SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR / BMC Res. Notes 4:263
El zaqu vel, P. / 2008 / A multiplex RT-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables / Food Microbiol 25:705~713
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