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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Min-Goo (Department of Biological Science and Technology, and Institute of Biomaterials, Yonsei University) Seo, Hee-Won (Department of Biological Science and Technology, and Institute of Biomaterials, Yonsei University) Choi, Yo-Han (Department of Biological Science and Technology, and Institute of Biomaterials, Yonsei University) Lee, Chang-Kyu (Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University) Ka, Hak-Hyun (Department of Biological Science and Technology, and Institute of Biomaterials, Yonsei University)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제24권 제2호
발행연도
2009.1
수록면
77 - 87 (11page)

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To understand molecular and cellular mechanisms of many gene products in the female reproductive organs including the ovary and uterine endometrium as well as during embryo development, researchers have developed and utilized many effective methodologies to analyze gene expression in cells, tissues and animals over the last several decades. For example, blotting techniques have helped to understand molecular functions at DNA, RNA and protein levels, and the reverse transcription-polymerase chain reaction (RT-PCR) method has been widely used in gene expression analysis. However, some conventional methods are not sufficient to understand regulation and function of genes expressed in very complex patterns in many organs. Thus, it is required to adopt more high-throughput and reliable techniques. Here, we describe several techniques used widely recently to analyze gene expression, including annealing control based-PCR, differential display-PCR, expressed sequence tag, suppression subtractive hybridization and microarray techniques. Use of these techniques will help to analyze expression pattern of many genes from small scale to large scale and to compare expression patterns of genes in one sample to another. In this review, we described principles of these methodologies and summarized examples of comparative analysis of gene expression in female reproductive organs with help of those methodologies.

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