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논문 기본 정보

자료유형
학술저널
저자정보
Park, Sang-Kyu (Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute and CLS21, Seoul National University School of Dentistry) Choi, Young-Ju (Seoul National University College of Medicine) Roh, Sang-Ho (Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute and CLS21, Seoul National University School of Dentistry)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제25권 제4호
발행연도
2010.1
수록면
259 - 262 (4page)

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Optimization of the preimplantation mammalian embryo culture condition was widely focused on refining medium composition under the name of chemically defined media. However, recent research revealed that the alteration of physical environment can be a crucial factor to a successful embryo development. In this study, under the same embryo density, a novel culture device named oil-free micro tube culture (MTC) system was evaluated using porcine parthenogenetic embryos. The activated oocytes were placed into the 0.2 ml thin-wall flat cap PCR tube and cultured to the blastocyst stage. As a preliminary step, embryo density and culture medium volume were optimized under a standard drop culture system. The optimal embryo density range for in vitro culture was 0.5 embryos per ${\mu}l$ in $20\;{\mu}l$ drop (20.5%) and 1.0 embryos per ${\mu}l$ in $10\;{\mu}l$ drop (20.6%). Based on these results, we compared drop culture system and 'MTC' system in terms of the developmental rate to the blastocyst stage. In $20\;{\mu}l$ medium volume, the 'MTC' system showed similar blastocyst formation rate when compared with drop culture system (20.2% versus 20.5%, respectively) while the 'MTC' system showed lower blastocyst formation rate than drop culture system in $10\;{\mu}l$ one (12.7% versus 20.0%, respectively). Therefore the $20\;{\mu}l$ MTC system may be an alternative incubation system for short-distance embryo transport without carrying the $CO_2$ incubator and this provides novel embryo culture device to clinical veterinary embryologists.

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