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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 62 - 67 (6page)
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TNF-related apoptosis-inducing ligand (TRAIL) is a promising agent for management of cancer because of its selective cytotoxicity to cancer cells. However, some cancer cells have resistance to TRAIL. Accordingly, novel treatment strategies are required to overcome TRAIL resistance. Here, we examined the synergistic apoptotic effect of apigenin in combination with TRAIL in Huh-7 cells. We found that combined treatment of TRAIL and apigenin markedly inhibited Huh-7 cell growth compared to either agent alone by inducing apoptosis. Combined treatment with apigenin and TRAIL induced chromatin condensation and the cleavage of poly (ADP-ribose) polymerase (PARP). In addition, enhanced apoptosis by TRAIL/apigenin combination was quantified by annexin V/PI flow cytometry analysis. Western blot analysis suggested that apigenin sensitizes cells to TRAIL-induced apoptosis by activating both intrinsic and extrinsic apoptotic pathway-related caspases. The augmented apoptotic effect by TRAIL/apigenin combination was accompanied by triggering mitochondria-dependent signaling pathway, as indicated by Bax/Bcl-2 ratio up-regulation. Our results demonstrate that combination of TRAIL and apigenin facilitates apoptosis in Huh-7 cells.
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참고문헌 (37)
참고문헌 신청
Adams, J. M. / 2007 / The Bcl-2 apoptotic switch in cancer development and therapy / Oncogene 26:1324~1337
Andrzejewski, T. / 2008 / Therapeutic effi cacy of curcumin/TRAIL combination regimen for hormone-refractory prostate cancer / Oncol. Res 17:257~267
Armeanu, S. / 2003 / Adenoviral gene transfer of tumor necrosis factor-related apoptosis-inducing ligand overcomes an impaired response of hepatoma cells but causes severe apoptosis in primary human hepatocytes / Cancer Res 63:2369~2372
Ashkenazi A / 1999 / Safety and antitumor activity of recombinant soluble Apo2 ligand / J. Clin. Invest 104:155~162
Bhojani, M. S. / 2003 / TRAIL and anti-tumor responses / Cancer Biol. Ther 2(4 Suppl 1):S71~S78
Andrzejewski, T. / 2008 / Therapeutic effi cacy of curcumin/TRAIL combination regimen for hormone-refractory prostate cancer / Oncol. Res 17:257~267
Armeanu, S. / 2003 / Adenoviral gene transfer of tumor necrosis factor-related apoptosis-inducing ligand overcomes an impaired response of hepatoma cells but causes severe apoptosis in primary human hepatocytes / Cancer Res 63:2369~2372
Ashkenazi A / 1999 / Safety and antitumor activity of recombinant soluble Apo2 ligand / J. Clin. Invest 104:155~162
Bhojani, M. S. / 2003 / TRAIL and anti-tumor responses / Cancer Biol. Ther 2(4 Suppl 1):S71~S78
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