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논문 기본 정보

자료유형
학술저널
저자정보
Hong, Joo-Heon (Department of Food Science and Technology, Catholic University of Daegu) Jung, Hee Kyoung (Biohealth Convergence Center, Daegu Technopark)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제57권 제1호
발행연도
2014.1
수록면
105 - 112 (8page)

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초록· 키워드

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${\beta}$-Glucan isolated from cell wall of fungi not only has low purity and yield, but also causes adverse effects. Consequently, extracellular ${\beta}$-glucan produced by microorganisms is the focus of this study. ${\beta}$-Glucan-rich exopolysaccharides (G-EPSs) with different molecular weights from Paenibacillus polymyxa JB115 were prepared by ultrafiltration and spray-drying. The weight-average molecular masses of the G-EPSs, P-SD-1 (spray-dried G-EPSs powder prepared below 100 kDa) and P-SD-2 (spray-dried G-EPSs powder prepared above 100 kDa), were $7.084{\times}10^4$ and $9.235{\times}10^5g/mol$, respectively. ${\beta}$-Glucan content was 47.38% in P-SD-1 and 73.12% in P-SD-2. The hydroxyl radical- and superoxide radical-scavenging activities of P-SD-2 at 1 mg/mL (39.45 and 87.34%, respectively) were higher than those of P-SD-1 (30.32 and 53.06%, respectively). Maximal nitric oxide ($22.24{\pm}1.34{\mu}M$) was generated in the presence of P-SD-2 (1 mg/mL) and the antitumor activity of P-SD-2 was higher than P-SD-1 in four tumor cell lines (HeLa, Sarcoma 180, A549, and Hep3B cells). Thus, antioxidant and antitumor activities could be enhanced by regulating the molecular weight of G-EPSs. We anticipate that the food and medicinal use of G-EPSs will follow further characterization of this class of exopolysaccharides.

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