지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 5 - 14 (10page)
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초록· 키워드
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A bacterial strain, designated as IJ-1, was newly isolated from crude oil sample collected in Taean oil spill contaminated area, Korea. The isolate had a biosurfactant-producing activity due to development of a hemolysis zone around the colony on blood agar plate. The strain was identified as Bacillus pumilus IJ-1 based on its morphological and biochemical characteristics. Phylogenetic analysis using 16S rDNA sequences confirmed the strain as B. pumilus IJ-1. Glycerol was the best carbon source for the biosurfactant production by B. pumilus IJ-1. The maximum production of the biosurfactant was obtained when tryptone was supplied as a nitrogen source. Biosurfactant production was associated with the growth of B. pumilus IJ-1. The yield of biosurfactant reached maximum level (2.6 g/L) during the stationary phase, during which the cell-free broth containing the biosurfactant could reduce the surface tension from 58.6 dyne/cm to 27.5 dyne/cm. B. pumilus IJ-1 was able to degrade crude oil, diesel, gasoline, and kerosene. The biosurfactant produced by B. pumilus IJ-1 showed the good emulsification activities on vegetable oils, paraffin, and crude oil. In particular, olive oil was the best substrate for emulsification activity. GC-FID analysis of the crude oil remaining in the culture medium showed that B. pumilus IJ-1 was almost completely degraded during incubation for 96 h.
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