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자료유형
학술저널
저자정보
Than, Van Thai (Department of Microbiology, Chung-Ang University College of Medicine) Baek, In-Hyuk (Department of Microbiology, Chung-Ang University College of Medicine) Lee, Hee-Young (Medikan Inc.) Kim, Jong-Bum (Functional Biomaterial Division, National Academy of Agricultural Science, the Rural Development Administration) Shon, Dong-Hwa (Food Immunology Research Group, Korea Food Research Institute) Chung, In-Sik (Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University) Kim, Won-Yong (Department of Microbiology, Chung-Ang University College of Medicine)
저널정보
한국응용약물학회 Biomolecules & Therapeutics(구 응용약물학회지) Biomolecules & therapeutics 제20권 제3호
발행연도
2012.1
수록면
320 - 325 (6page)

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Rotavirus and hepatitis A virus (HAV) spread by the fecal-oral route and infections are important in public health, especially in developing countries. Here, two antigenic epitopes of the HAV polyprotein, domain 2 (D2) and domain 3 (D3), were recombined with rotavirus VP7, generating D2/VP7 and D3/VP7, cloned in a baculovirus expression system, and expressed in Spodoptera frugiperda 9 (Sf9) insect cells. All were highly expressed, with peak expression 2 days post-infection. Western blotting and ELISA revealed that two chimeric proteins were antigenic, but only D2/VP7 was immunogenic and elicited neutralizing antibody responses against rotavirus and HAV by neutralization assay, implicating D2/VP7 as a multivalent subunit-vaccine Candidate for preventing both rotavirus and HAV infections.

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