지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 949 - 958 (10page)
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초록· 키워드
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Fisetin, a flavonol, has been known as an anti-allergic agent having inhibitory effects on T helper 2 cytokine gene expression in inflammatory immune cells. However, its molecular mechanisms for suppressive effects of fisetin on interleukin (IL)-4 and IL-13 in activated mast cells and basophils have been incompletely understood. In this study we found that fisetin at the concentrations having no effect on cell viability significantly inhibited the phorbol 12-myristate 13-acetate and ionomycin induced production of IL-4 and IL-13 in bone marrow-derived mast cells and RBL-2H3 basophilic cells. The levels of mRNA were dramatically decreased by fisetin, indicating the suppression might be regulated at the transcriptional levels. Transient transfection experiments using luciferase reporter plasmids expressing IL-4 or IL-13 promoter revealed that fisetin inhibited the activation of the promoters in a dose-dependent manner. Western blot analysis of the nuclear expression of various transcription factors involved in the promoter activation indicated that suppressions of c-Fos and CCAAT/enhancer-binding protein alpha were prominent together with significant down-regulations of nuclear factor of activated T cell (NF-AT) and NF-${\kappa}B$. Furthermore, nuclear expression of GATA-1 and GATA-2, and the mRNA expression were significantly down regulated by fisetin, whereas cyclosporine A had no significant effects on GATA transcription factors. Taken together, fisetin has suppressive effects on IL-4 and IL-13 gene expressions through the regulation of selective transcription factors.
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