지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 99 - 104 (6page)
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초록· 키워드
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Pseudomonas tolaasii causes brown blotch disease in cultivated mushrooms by producing tolaasin, a peptide toxin, which forms pores on the membrane and disrupts the cellular and fruiting body structures of mushrooms. For the biological control of this mushroom disease, virulent bacteriophages of P. tolaasii were isolated from the sewage of Cheongju, Korea. Twenty-one phages were isolated from four different locations, and their toxicities to host bacteria were measured by inspecting the turbidity and size of their plaques. They were divided into three categories on the basis of their toxicities to host bacteria. In order to test if these phages can be used for the biological control of mushroom diseases, a pitting test was performed. The surfaces of mushroom caps were inoculated with both pathogenic bacteria and their phages. Phage toxicity was analyzed by measuring the size of the blotches that formed on the surface of mushrooms, because these sizes are representative of the amount of tolaasin peptide produced by pathogenic bacteria in the presence of bacteriophages. The formation of blotches was completely blocked by co-incubated phages. These results show that phages can sterilize pathogenic bacteria in mushroom tissues as well as be useful for the biological control of brown blotch disease. The optimum conditions for the bactericidal activity of the phages were also determined.
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