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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Ji Young (Research Center for Radiotherapy, Korea Institute of Radiological and Medical Sciences,) Chung, Namhyun (Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University) Lee, Yong Kwon (Department of Food and Nutrition, Yuhan College)
저널정보
한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제57권 제1호
발행연도
2014.1
수록면
61 - 63 (3page)

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Aged black garlic (ABG) was extracted with 20% ethanol and water (crude extracts) and fractionated into three categories (>10, 3-10, and <3 kDa). The effect of crude extract supplements on anti-My-10 hybridoma cell growth and IgG1 antibody production was investigated in suspension culture with a chemically defined protein-free medium. We observed that supplementation of ABG to the cell culture medium stimulated anti-My-10 hybridoma cell growth and production of IgG1 antibody, particularly with fractionated ABG of low molecular weight. The stimulation depended upon the concentration and the size of the fractionated ABG. We also found that the growth-promoting activity was not correlated with high antibody production. These results suggest that fractionated ABG is a novel and promising alternative as an animal cell culture supplement.

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