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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제57권 제2호
- 발행연도
- 2014.1
- 수록면
- 103 - 111 (9page)
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초록· 키워드
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A1E is an extract from traditional Asian medicinal plants that has therapeutic activities against cancers, metabolic disease, and other intractable conditions. However, its mechanism of action on cervical cancer has not been studied. In order to ascertain if A1E would have pronounced anti-cervical cancer effect, cervical cancer cells were incubated with A1E and apoptosis was detected by nuclear morphological changes, annexin V-FITC/PI staining, cell cycle analysis, western blotting, Reverse-transcription polymerase chain reaction, and measurement of mitochondrial membrane potential. Expression of human papiloma virus E6 and E7 oncogenes was down-regulated in A1E-treated cervical cancer cells, while p53 and retinoblastoma protein levels were enhanced. A1E also perturbed cell cycle progression at sub-G1 and altered cell cycle regulatory factors in SiHa cervical cancer cells. A1E activated apoptotic intrinsic pathway markers such as caspase-9, caspase-3 and poly ADP-ribose polymerase, and down-regulated expression of Bcl-2 and Bcl-xl. A1E induced mitochondrial membrane potential collapse and cytochrome c release, and inhibited phosphatidylinositol 3-kinase (PI3K)/Akt, key factors involved in cell survival signaling. Taken all these results, A1E induced apoptosis via activation of the intrinsic pathway and inhibition of the PI3K/Akt survival-signaling pathway in SiHa cervical cancer cells. In conclusion, A1E exerts anti-proliferative action growth inhibition on cervical cancer cells through apoptosis which demonstrates its anti-cervical cancer properties.
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참고문헌 (34)
참고문헌 신청
Bak Y / 2013 / A1E inhibits proliferation and induces apoptosis in NCI-H460 lung cancer cells via extrinsic and intrinsic pathways / Mol Biol Rep 40:4507~4519
Bak YS / 2011 / A synthetic naringenin derivative, 5-hydroxy-7, 4'-diacetyloxyflavanone-Nphenyl hydrazone(N101-43), induces apoptosis through up-regulation of Fas/FasL expression and inhibition of PI3K/Akt signaling pathways in non-small-cell lung cancer cells / J Agric Food Chem 59:10286~10297
Chang F / 2003 / Involvement of PI3K/Akt pathway in cell cycle progression, apoptosis, and neoplastic transformation: a target for cancer chemotherapy / Leukemia 17:590~603
Chen J / 2012 / Structure-Based Discovery of BM-957 as a Potent Small-Molecule Inhibitor of Bcl-2 and Bcl-xL Capable of Achieving Complete Tumor Regression / J Med Chem 55:8502~8514
Doorbar J / 2006 / Molecular biology of human papillomavirus infection and cervical cancer / Clin Sci (Lond) 110:525~541
Bak YS / 2011 / A synthetic naringenin derivative, 5-hydroxy-7, 4'-diacetyloxyflavanone-Nphenyl hydrazone(N101-43), induces apoptosis through up-regulation of Fas/FasL expression and inhibition of PI3K/Akt signaling pathways in non-small-cell lung cancer cells / J Agric Food Chem 59:10286~10297
Chang F / 2003 / Involvement of PI3K/Akt pathway in cell cycle progression, apoptosis, and neoplastic transformation: a target for cancer chemotherapy / Leukemia 17:590~603
Chen J / 2012 / Structure-Based Discovery of BM-957 as a Potent Small-Molecule Inhibitor of Bcl-2 and Bcl-xL Capable of Achieving Complete Tumor Regression / J Med Chem 55:8502~8514
Doorbar J / 2006 / Molecular biology of human papillomavirus infection and cervical cancer / Clin Sci (Lond) 110:525~541
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