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학술저널
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Woo, Hee-Jong (National Academy of Agricultural Science, Rural Development Administration [RDA]) Chung, Chan-Mi (National Academy of Agricultural Science, Rural Development Administration [RDA]) Shin, Kong-Sik (National Academy of Agricultural Science, Rural Development Administration [RDA]) Lim, Myung-Ho (National Academy of Agricultural Science, Rural Development Administration [RDA]) Lee, Ki-Jong (National Academy of Agricultural Science, Rural Development Administration [RDA]) Cho, Yong-Gu (Department of Crop Science, Chungbuk National University) Kweon, Soon-Jong (National Academy of Agricultural Science, Rural Development Administration [RDA]) Suh, Seok-Cheol (National Academy of Agricultural Science, Rural Development Administration [RDA])
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제54권 제6호
발행연도
2011.1
수록면
860 - 864 (5page)

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A novel genetically modified organism (GMO) detection method was developed using a simple and rapid procedure to identify a regulatory gene introduced into genetically modified (GM) maize. After DNA extraction from GM maize flours, fragments of the conserved sequence of the cauliflower masaic virus (CaMV) 35S promoter gene along with a competitive internal control gene were amplified using duplex polymerase chain reaction (PCR). Amplification products were then detected using a disposable detection device. The quantitative detection limit for GM maize 59122 was found to be 1.0% (w/w). Because the combination-method involving PCR technology coupled with a disposable detection device does not require expensive instrumentation or expertise, it will serve as a valuable alternative to immunoassays and traditional PCR-based tests in the detection of GMOs.

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