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자료유형
학술저널
저자정보
Chukeatirote, Ekachai (Institute of Excellence in Fungal Research, Mae Fah Luang University) Maharachchikumbura, Sajeewa S.N. (Institute of Excellence in Fungal Research, Mae Fah Luang University) Wongkham, Shannaphimon (Scientific & Technological Instruments Centre, Mae Fah Luang University) Sysouphanthong, Phongeun (Institute of Excellence in Fungal Research, Mae Fah Luang University) Phookamsak, Rungtiwa (Institute of Excellence in Fungal Research, Mae Fah Luang University) Hyde, Kevin D. (Institute of Excellence in Fungal Research, Mae Fah Luang University)
저널정보
한국균학회 Mycobiology Mycobiology 제40권 제2호
발행연도
2012.1
수록면
107 - 110 (4page)

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초록· 키워드

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Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems. The sizes of these PCR amplicons were between 700~800 bp. The DNA sequences obtained were similar showing high identity to the cbhI gene family. These cbhI genes were partial consisting of three coding regions and two introns. The deduced amino acid sequences exhibited significant similarity to those of fungal CBHI enzymes belonging to glycosyl hydrolase family 7.

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