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자료유형
학술저널
저자정보
Park, Ah Reum (IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology [KITECH]) Park, Jeong-Hoon (IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology [KITECH]) Ahn, Hye-Jin (IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology [KITECH]) Jang, Ji Yeon (IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology [KITECH]) Yu, Byung Jo (IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology [KITECH]) Um, Byung-Hwan (Department of Chemical Engineering, Hankyong National University) Yoon, Jeong-Jun (IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology [KITECH])
저널정보
한국균학회 Mycobiology Mycobiology 제43권 제1호
발행연도
2015.1
수록면
57 - 62 (6page)

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${\beta}$-Glucosidase, which hydrolyzes cellobiose into two glucoses, plays an important role in the process of saccharification of the lignocellulosic biomass. In this study, we optimized the activity of ${\beta}$-glucosidase of brown-rot fungus Fomitopsis pinicola KCTC 6208 using the response surface methodology (RSM) with various concentrations of glucose, yeast extract and ascorbic acid, which are the most significant nutrients for activity of ${\beta}$-glucosidase. The highest activity of ${\beta}$-glucosidase was achieved 3.02% of glucose, 4.35% of yeast extract, and 7.41% ascorbic acid where ascorbic acid was most effective. The maximum activity of ${\beta}$-glucosidase predicted by the RSM was 15.34 U/mg, which was similar to the experimental value 14.90 U/mg at the 16th day of incubation. This optimized activity of ${\beta}$-glucosidase was 23.6 times higher than the preliminary activity value, 0.63 U/mg, and was also much higher than previous values reported in other fungi strains. Therefore, a simplified medium supplemented with a cheap vitamin source, such as ascorbic acid, could be a cost effective mean of increasing ${\beta}$-glucosidase activity.

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