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논문 기본 정보

자료유형
학술저널
저자정보
Vu, Van Hanh (Department of Bioscience and Biotechnology, The University of Suwon) Pham, Tuan Anh (Department of Bioscience and Biotechnology, The University of Suwon) Kim, Keun (Department of Bioscience and Biotechnology, The University of Suwon)
저널정보
한국균학회 Mycobiology Mycobiology 제39권 제1호
발행연도
2011.1
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20 - 25 (6page)

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Spores of Aspergillus sp. SU14 were treated repeatedly and sequentially with $Co^{60}$ ${\gamma}$-rays, ultraviolet irradiation, and N-methyl-N'-nitro-N-nitrosoguanidine. One selected mutant strain, Aspergillus sp. SU14-M15, produced cellulase in a yield 2.2-fold exceeding that of the wild type. Optimal conditions for the production of cellulase by the mutant fungal strain using solid-state fermentation were examined. The medium consisted of wheat-bran supplemented with 1% (w/w) urea or $NH_4Cl$, 1% (w/w) rice starch, 2.5 mM $MgCl_2$, and 0.05% (v/w) Tween 80. Optimal moisture content and initial pH was 50% (v/w) and 3.5, respectively, and optimal aeration area was 3/100 (inoculated wheat bran/container). The medium was inoculated with 25% 48 hr seeding culture and fermented at $35^{\circ}C$ for 3 days. The resulting cellulase yield was 8.5-fold more than that of the wild type strain grown on the basal wheat bran medium.

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