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학술저널
저자정보
Kang, Eun-Hye (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) Song, Eun-Jung (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) Kook, Jun Ho (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) Lee, Hwan-Hee (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) Jeong, Bo-Ri (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) Park, Hee-Moon (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
저널정보
한국균학회 Mycobiology Mycobiology 제43권 제1호
발행연도
2015.1
수록면
31 - 36 (6page)

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We have previously isolated ${\varepsilon}$-COP, the ${\alpha}$-COP interactor in COPI of Aspergillus nidulans, by yeast two-hybrid screening. To understand the function of ${\varepsilon}$-COP, the $aneA^+$ gene for ${\varepsilon}$-COP/AneA was deleted by homologous recombination using a gene-specific disruption cassette. Deletion of the ${\varepsilon}$-COP gene showed no detectable changes in vegetative growth or asexual development, but resulted in decrease in the production of the fruiting body, cleistothecium, under conditions favorable for sexual development. Unlike in the budding yeast Saccharomyces cerevisiae, in A. nidulans, over-expression of ${\varepsilon}$-COP did not rescue the thermo-sensitive growth defect of the ${\alpha}$-COP mutant at $42^{\circ}C$. Together, these data show that ${\varepsilon}$-COP is not essential for viability, but it plays a role in fruiting body formation in A. nidulans.

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