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논문 기본 정보

자료유형
학술저널
저자정보
Chung, Jun-Young (Department of Anesthesiology, College of Medicine, Kyung Hee University) Yi, Jae-Woo (Department of Anesthesiology, College of Medicine, Kyung Hee University) Kim, Sung-Min (Department of Neurosurgery, College of Medicine, Kyung Hee University) Lim, Young-Jin (Department of Neurosurgery, College of Medicine, Kyung Hee University) Chung, Joo-Ho (Department of Pharmacology, College of Medicine, Kyung Hee University) Jo, Dae-Jean (Department of Neurosurgery, College of Medicine, Kyung Hee University)
저널정보
대한신경외과학회 대한신경외과학회지 대한신경외과학회지 제50권 제3호
발행연도
2011.1
수록면
173 - 178 (6page)

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Objective : The rat middle cerebral artery thread-occlusion model has been widely used to investigate the pathophysiological mechanisms of stroke and to develop therapeutic treatment. This study was conducted to analyze energy metabolism, apoptotic signal pathways, and genetic changes in the hippocampus of the ischemic rat brain. Methods : Focal transient cerebral ischemia was induced by obstructing the middle cerebral artery for two hours. After 24 hours, the induction of ischemia was confirmed by the measurement of infarct size using 2,3,5-triphenyltetrazolium chloride staining. A cDNA microarray assay was performed after isolating the hippocampus, and was used to examine changes in genetic expression patterns. Results : According to the cDNA microarray analysis, a total of 1,882 and 2,237 genes showed more than a 2-fold increase and more than a 2-fold decrease, respectively. When the genes were classified according to signal pathways, genes related with oxidative phosphorylation were found most frequently. There are several apoptotic genes that are known to be expressed during ischemic brain damage, including Akt2 and Tnfrsf1a. In this study, the expression of these genes was observed to increase by more than 2-fold. As energy metabolism related genes grew, ischemic brain damage was affected, and the expression of important genes related to apoptosis was increased/decreased.Conclusion : Our analysis revealed a significant change in the expression of energy metabolism related genes (Atp6v0d1, Atp5g2, etc.) in the hippocampus of the ischemic rat brain. Based on this data, we feel these genes have the potential to be target genes used for the development of therapeutic agents for ischemic stroke.
#Apoptosis #Energy metabolism #Focal cerebral ischemia #Gene expression #Hippocampus #Oxidative phosphorylation

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참고문헌 (28)

참고문헌 신청
Alkayed NJ / 2000 / Neuroprotective effects of female gonadal steroids in reproductively senescent female rats / Stroke 31:161~168 google schola Bederson JB / 1986 / Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats / Stroke 17:1304~1308 google schola Chen A / 2007 / Upregulation of dihydropyrimidinase- related protein 2, spectrin alpha II chain, heat shock cognate protein 70 pseudogene 1 and tropomodulin 2 after focal cerebral ischemia in rats--a proteomics approach / Neurochem Int 50:1078~1086 google schola Dugan LL / 1994 / Excitotoxicity, free radicals, and cell membrane changes / Ann Neurol 35:17~S21 google schola Fonteh AN / 2006 / Identification of disease markers in human cerebrospinal fluid using lipidomic and proteomic methods / Dis Markers 22:39~64 google schola

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